ABSTRACT
Objective To found sensitive and reliable method to identify trophoblastic tumor cells in the peripheral blood of the patients suffered from gestational trophoblastic tumor.Methods Given numbers of JAR cell from ten to million were mixed into 10ml non pregnant peripheral blood as a model. Detection of ? hCG mRNA with fluorescence quantitative reverse transcription polymerase chain reaction (FQ RT PCR) and then estimation of the numbers of tumor cell in the blood. Nine cases of peripheral blood were collected from the pretreatment patients of gestational trophoblastic tumor to assay ? hCG mRNA with FQ RT PCR, then to estimate the numbers of tumor cell in the circulation blood. Results FQ RT PCR could detect ? hCG mRNA when ≥10 2 JAR cells were mixed into 10ml non pregnant peripheral blood. Four cases of bloods had been detected ? hCG mRNA expression in 9 cases of gestational trophoblastic tumor, and the numbers of tumor cell from 10 4 to 10 7 per 10ml blood. Conclusion FQ RT PCR of which primers and probe are designed for ? hCG had been proved to be very sensitive detection means, it could be used to detect gestational trophoblastic tumor cells from patient preipheral blood; With FQ RT PCR the tumor cells had been detected in some patients of gestational trophoblastic tumor.
ABSTRACT
Objective To investigate the possibility that TGF ? may also influence the basal and FSH stimulated production of estradiol and progesterone by granulosa cells in vitro. Methods Granulosa cells were obtained from infertile patients undergoing in vitro fertilization and embryo transfer treatment and cultured with serum free supplemented HAM′s F 10 medium(control) or increasing concentrations of TGF ? 1,2 in the absence or presence of FSH (96 U/ml). The media were collected in 24, 48, 72 and 96 h intervals and assayed for estradiol and progesterone. Results Increasing amounts of TGF ? had a significant effect on estradiol production, especially in the presence of FSH,The estradiol value was evaluated from (12 077.9?5 835.3) pmol/L and (13 656.1? 8 404 3) pmol/L to (16 606.8?7 993.3) pmol/L and (19 480.4?913.8) pmol/L. TGF ? inhibited FSH stimulated progesterone but not unstimulated progesterone release ( P
ABSTRACT
AIM: To investigate the growth inhibition of Candida albicans mediated by vaginal epithelial cells and determine if estrogen affects the anti-fungal activity. METHODS: C_ 57BL/6J mice vaginae from estrogen treatment group, diestrus group and ovariectomized group were excised, respectively. The vaginae were dissociated into single cell suspension by dispase and collagenaseⅠ. The epithelial nenriched cells were used as effector cells. Blastoconidia of C. albicans were used as target cells. After coincubation of effector with target for 9 h, the target cells growth density was observed, percent growth inhibition was calculated, and ultra-structural changes were observed. RESULTS: After coincubation of effector cells with target cells for 9 h, growth density of C. albicans was visibly reduced and it's growth activity was inhibited. Compared to ovariectomized group and diestrus group, vaginal epithelial cells from estrogen-treated mice had less ability to inhibit the growth of C. albicans (P