Ecological study of rotifers from Kor river, Fars, Iran.

In the present study, water samples from 3 stations of Kor river, Iran were analyzed for seasonal fluctuation of physico-chemical parameters, i.e. air and water temperature, dissolved oxygen, BOD, COD, total nitrogen, total hardness, pH, alkalinity, acidity, total dissolved solid and water current. Effect of physico-chemical parameters on surface water density of rotifers was also studied. During one year study 13 species of rotifers belonging to 2 classes and 3 orders, 6 families and 10 genera were recognized. Maximum population density belongs to Lecane luna and the minimum population density was observed in Keratella valga and Monostyla lunaris. An inverse relationship between the population density of rotifera and the water current as wall as a direct relationship between the water temperature and the population density of rotifers was observed in all sampling stations and throughout the study period.

Evaluation of cellular toxicityfor cisplatin, arsenic and a-cetaminophen in the cancer and normal cell line

Cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. Today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components. Clonogenic assay was used for assessment of cell toxicity and amount of cell death after a specific time during which cells were exposed to different compounds. Thus, IC50 in caner cell lines [HePG2, SKOV3 and A549] and normal cell [LLCPK1, CHO and HGF1] was assessed after exposure to cisplatin, acetaminophen and arsenic. Results showed that acetaminophen has maximum resistance and minimum sensitivity in CHO line with IC50 = 16.7 +/- 1.06 HePG2 with IC50 = 18.6 +/- 1.29. On the other hand, cisplatin showed minimum resistance and maximum sensitivity in HePG2 with IC50 = 0.87 +/- 0.07 and HGF1 with IC50 = 1.6 +/- 0.21 and lastly, arsenic showed minimum resistance and maximum sensitivity in A549 with IC50 = 4.59 +/- 0.29 and LLCPK1 with IC50-1 +/- 0.37. According to the evaluated IC50, there were differences between results of sensitivity of cell lines exposed to the three drugs [P < 0.05]. Entirely, resistance in cancer cell lines was lower than normal cells. The results showed the importance of cell defensive mechanisms encountering different substances like glutathione