ABSTRACT
Background: The aim of present study was to clarify the role of the peroxisome proliferator-activated receptor [PPAR] gamma Pro12Ala and C161T polymorphisms in the pathogenesis of polycystic ovary syndrome [PCOS] and their influence on lipid and lipoprotein profiles of patients
Materials and Methods: The present cross-sectional study consisted of 50 women with PCOS, who referred to the Kermanshah University of Medical Sciences Clinic between April and October 2015, and 233 unrelated age-matched healthy women from the same region [West Iran]. The PPAR gamma Pro12Ala and PPAR gamma C161T polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method. Fasting blood sugar [FBS], serum triglycerides [TG], cholesterol, low density lipoprotein- cholesterol [LDL-C], high density lipoprotein- cholesterol [HDL-C] and estradiol levels were measured
Results: The serum level of estradiol was significantly lower in PCOS patients compared to healthy women. The PPAR gamma Pro12Ala [CG] genotype increased the risk of PCOS 2.96-fold. The frequency of the PPAR gamma T allele [at C161T] was 21% in patients and 17.2% in controls with no significant difference [P=0.52]. In all studied individuals, the PPARgamma CG genotype was associated with significantly higher levels of TG. However, significantly lower levels of total cholesterol and LDL-C were observed in PPAR gamma TT individuals compared with those with the CC genotype. Within the PCOS group, the PPAR gamma CG genotype was significantly associated with lower levels of estradiol compared with the CC genotype. Also, the CG genotype was significantly associated with higher levels of TG when compared with the CC genotype
Conclusion: Our study shows that, unlike PPAR gamma C161T, PPAR gamma Pro12Ala is associated with the risk of PCOS. Also, we found that the lipid and lipoprotein profiles significantly vary based on PPAR gamma Pro12Ala and C161T genotypes