ABSTRACT
@#Introduction: Diabetes mellitus (DM) is a disease that can cause complications in the kidneys. Centella asiatica extracts have the potential to inhibit pancreatic, liver and kidney tissue damage. This study was intended to determine the potential of C. asiatica extract in inhibiting kidney damage in an animal model of DM. Methods: Male Wistar rats were used in 5 treatment groups namely non-DM, DM, and DM with C. asiatica extract Dose 1 (250mg / kg), Dose 2 (500mg / kg) and Dose 3 (1000mg / kg). Changes in body weight, blood sugar, serum urea, kidney weight, glycosuria, and urine volume were observed in all treatment groups. Results: There were no significant differences between treatment groups on changes in blood glucose concentration, body weight, and serum ureum. However, C. asiatica treated group showed significantly lower value of urine volume, glycosuria, and kidney weight compare to those on Non-DM and DM groups. Decrease in blood glucose, although not significantly different, affects glucose urine excretion. Conclusion: C. asiatica extract has the potential to inhibit kidney damage in rats with DM through prevent the increase of urine volume, glycosuria, and kidney weight.
ABSTRACT
In previous study, in vitro antiplasmodial activity fractions isolated from methanol extract of E. longifolia, Jack. have been evaluated. Among 5 isolates evaluated from the study, isolate 4 showed high in vitro antiplasmodial activity. However, which stage specificity of the isolates on P. falciparum cycles has not been evaluated. This study was intended to evaluate the stage specificity of the isolate on P. falciparum cycles. The study was conducted by observing the percentage of each stages of P. falciparum microscopically after 8, 16, 24, 32, 40, 48, 56, 64, and 72 hours incubation periods with 3 various concentration of isolate 4 compared with control. The result showed that isolate 4 of E. longifolia root methanol soluble fractions most potent at trophozoites stages of P. falciparum.