ABSTRACT
AIM@#To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum.@*METHOD@#The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively.@*RESULTS@#Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A (1), microintegerrin B (2), scopoletin (3), and scopolin (4). All of the compounds were tested for their cytotoxicity against three cancer cell lines (HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus.@*CONCLUSION@#Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 μg·mL(-1).
Subject(s)
Humans , Candida albicans , Coumarins , Pharmacology , Glucosides , Pharmacology , HeLa Cells , Molecular Structure , Phenylpropionates , Chemistry , Pharmacology , Plant Extracts , Chemistry , Pharmacology , Plant Leaves , Plant Stems , Rutaceae , Chemistry , Scopoletin , Pharmacology , Staphylococcus aureusABSTRACT
Eleven compounds were isolated and purified from the barks extract of Nothopanax delavayi and their structures were identified as serratagenic acid-3-O-alpha-L-arabinopyranosyl-28-O-beta-D-glucopyranosyl ester (1), serratagenic acid-3-0-alpha-L-arabi-nopyranosyl-28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (2), serratagenic acid (3), serratagenic acid-3-O-alpha-L-arabinopyranoside (4), serratagenic acid-beta-O-beta-(2', 4'-O-diacetyl) -D-xylopyranosyl-28-O-[alpha-L-rhamnopy-ranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->46)-beta-D-glucopyranosyl] ester (5), serratagenic acid-3-O-alpha-(4'-O-acetyl)-L-arabino pyrano-syl-28-0- [-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(6), serratagenic acid-3-O-alpha-(2'-O-acetyl)-L-arabinopyranosyl-28-O-[-alpha-L-rhamnopyranosyl- (1-->4) -beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(7), serratagenic acid-3-0-beta-D-xylopyranosyl-28-O-[-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (8), protocatechuic acid (9), ethyl caffeate (10) and caffeic anhydride (11) by physicochemical properties and spectroscopic data analysis. Among them, compounds 3-4 and 9-11 were firstly isolated from the genus Nothopanax, and compounds 5-8 were isolated from this plant for the first time.
Subject(s)
Araliaceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Bark , ChemistryABSTRACT
Fourteen compounds were isolated and purified from the ethyl acetate of the ethanol extract of Shiaria bambusicola by various chromatographic methods, and their structures were elucidated by spectral techniques and physicochemical properties as hypocrellin A (1), hypocrellin B (2), hypocrellin C (3), hypomycin A (4), ergosterol (5), ergosterol peroxide (6), (22E, 24R)-5alpha, 8alpha-epidioxy-6,9(11),22-trien-3beta-ol (7), ergosta-7, 24(28)-dien-3beta-ol (8), (22E, 24R)-ergost-7, 22-dien-3beta, 5alpha, 6beta-triol (9), (22E,24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-3-O-palmitate (10), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-6-O-palmitate (11), 1-O-monostearin (12), 1, 3-O-distearin (13), and mannitol (14). Among them, compounds 7-13 were firstly isolated from this genus.
Subject(s)
Acetates , Chemistry , Ascomycota , Chemistry , Biological Factors , Chemistry , Molecular Structure , Spectrometry, Mass, Electrospray IonizationABSTRACT
To investigate the chemical constituents of the leaves of Adinandra nitida, several column chromatography methods were used to isolate the chemical constituents of this plant. The structures were elucidated on the basis of spectral data. Six compounds were isolated and identified as 2alpha, 3alpha, 19alpha-trihydroxy-olean-12-en-28-oic acid-28-O-beta-D-glucopyranoside (1), arjunetin (2), sericoside (3), glucosyl tormentate (4), nigaichigoside F1 (5) and arjunglucoside I (6), separately. Compound 1 is a new compound, and compounds 2 -6 were isolated from A. nitida for the first time.
Subject(s)
Glucosides , Chemistry , Molecular Structure , Oleanolic Acid , Chemistry , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Saponins , Chemistry , Theaceae , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of the root barks of Periploca sepium.</p><p><b>METHOD</b>Column chromatographic techniques were used to isolate the chemical constituents. NMR and MS methods were employed for their structural elucidation.</p><p><b>RESULT</b>Eight compounds were isolated and identified as isovanillin (1), vanillin (2), 4-methoxysalicylic acid (3), (24R)-9, 19-cycloart-25-ene-3beta, 24-diol (4), (24S)-9, 19-cycloart-25-ene-3beta, 24-diol (5), cycloeucalenol (6), beta-amyrin acetate (7) and alpha-amyrin (8).</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from this plant for the first time.</p>
Subject(s)
Benzaldehydes , Chemistry , Molecular Structure , Periploca , Chemistry , Phytosterols , Chemistry , Plant Bark , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents from flowers of Sesamum indicum.</p><p><b>METHOD</b>Column chromatography with silica gel, C18 and Sephadex LH -20 as packing materials was used to separate the chemical constituents, and the structures were determined by chemical and spectroscopic methods.</p><p><b>RESULT</b>Six flavones were isolated and elucidated as apigenin (1), ladanetin (2), ladanetin-6-O-beta-D-glucoside (3), apigenin-7-O-glucuronic acid (4), pedalitin (5), and pedalitin-6-O-glucoside (6).</p><p><b>CONCLUSION</b>All of the compounds were isolated from this plant for the first time.</p>
Subject(s)
Apigenin , Chemistry , Flavones , Chemistry , Flowers , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Sesamum , ChemistryABSTRACT
Sesame (Sesamum indicum L. ) belongs to Pedaliaceae, and its dry flowers have been used to cure alopecia, frostbite and constipation as a Traditional Chinese Medicine. Interestingly, the Flos Sesamum indicum L. was usually used to cure verruca vulgaris and verruca plana in folk of China, and showed a pleasant result. Previous chemical investigations of this plant mainly concentrate on its seeds, showed the presence of proteins and fat oils, herein we make a systematic chemical research on the dry flowers of this plant. Column chromatography including silica gel, C18 and Sephadex LH-20 were used to separate the chemical constituents and the structures were determined by chemical and spectroscopic methods. Ten compounds were isolated from the 95% ethanol extract of the plant and elucidated as latifonin (1), momor-cerebroside (2), soya-cerebroside II (3), 1-O-beta-D-glucopyranosyl-(2S, 3S, 4R, 5E,9Z)-2-N-(2'-hydroxytetracosanoyl) 1,3,4-trihydroxy-5,9-octadienine (4), 1-O-beta-D-glucopyranosyl-(2S, 3S, 4R, 8Z)-2-N-(2' R) 2'-hydroxytetracosanoyl) 3,4-dihydroxy-8-octadene (5), (2S, 1" S) -aurantiamide acetate (6), benzyl alcohol-O-(2'-O-beta-D-xylopyranosyl, 3'-O-beta-D-glucopyranoside)-beta-D-glucopyranoside (7), beta-sitosterol (8), daucosterol (9) and D-galacititol (10). Among them, 4 is a new compound, and others were isolated from the flowers of the plant for the first time. Compounds 2 to 4 belong to cerebroside, which is rare to be found in land plants and was proved to possess many bioactivities.
Subject(s)
Cerebrosides , Chemistry , Flowers , Chemistry , Glycolipids , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Sesamum , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents from the branch of Broussonetia papyrifera.</p><p><b>METHOD</b>Column chromatographic methods were used to isolate the chemical constituents. ESI-MS and NMR methods were employed for their structural elucidation.</p><p><b>RESULT</b>Six compounds were isolated and identified as (2S)-7, 3'-dihydroxy-4'-methoxyflavan (1), ergosterol peroxide (2), D-galacitol (3), sulfuretin (4), liriodendrin (5), graveolone (6), respectively.</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from the plant for the first time.</p>
Subject(s)
Benzofurans , Chemistry , Broussonetia , Chemistry , Ergosterol , Chemistry , Flavonoids , Chemistry , Furans , Chemistry , Glucosides , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of the stems and leaves of Aconitum coreanum (Lèvl.) Rapaics.</p><p><b>METHODS</b>The constituents of Aconitum coreanum were isolated by using various kinds of modern chromatographic methods. The new alkaloid was identified on the basis of spectral analysis.</p><p><b>RESULTS</b>Two compounds were isolated and identified as: 13-dehydro-1beta-acetyl-2alpha,6beta-dihydroxyhetisine (I) and Guanfu base G (II).</p><p><b>CONCLUSION</b>Compound I is a new alkaloid.</p>
Subject(s)
Aconitum , Chemistry , Diterpenes , Heterocyclic Compounds, Bridged-Ring , Chemistry , Molecular Conformation , Molecular Structure , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from rhizome of Pulsatilla dahurica.</p><p><b>METHOD</b>The constituents were isolated and purified by various chromatographic methods. AR compounds were identified on the basis of spectral analysis and physico-chemical characters.</p><p><b>RESULT</b>Six compounds were isolated from the 70% alcohol extract of the rhizome identified as hederagenin ( I ), hederagenin 3-O-alpha-L-arabinopyranoside (II), hederagenin 3-O-beta-D-glucopyranosyl(1-->2)-alpha-L-arabinopyranoside (III), hederagenin 3-O-beta-D-glucopyranosyl(1 -->2) [beta-D-glucopyranosyl(1-->4)]-alpha-L-arabinopyranoside (IV), beta-sitosterol (V) and daucosterol (VI), respectively.</p><p><b>CONCLUSION</b>Compounds I approximately VI were isolated from this plant for the first time.</p>
Subject(s)
Oleanolic Acid , Chemistry , Plants, Medicinal , Chemistry , Pulsatilla , Chemistry , Rhizome , Chemistry , Saponins , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Lygodium japonicum.</p><p><b>METHOD</b>Column chromatographic techniques were used for isolation and purification of chemical constituents of this plant and their structures were identified by spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and identified as tilianin (I), kaempferol-7-O-alpha-L-rhamnopyranoside (II), kaempferol (III), p-coumaric acid (IV), hexadecanoic acid 2, 3-dihydroxy-propyl ester (V), daucosterol (VI), beta-sitosterol (VII, and 1-hentriacontanol (VIII) respectively.</p><p><b>CONCLUSION</b>Compounds I, II, V, VI, VII, VIII were isolated from L. japonicum for the first time, compounds I, II, V were isolated from genus Lygodium for the first time.</p>
Subject(s)
Ferns , Chemistry , Flavonoids , Chemistry , Glycosides , Chemistry , Kaempferols , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the phenylethanoid glycosides from root of Picrorhiza scrophulariiflora.</p><p><b>METHOD</b>Column chromatographic techniques were used for isolation and purification of chemical constituents of the plant and the structures were identified by spectroscopic analysis.</p><p><b>RESULT</b>Six phenylethanoid glycosides were isolated and elucidated as: 2-(3,4-dihydroxyphenyl)-ethyl-O-beta-D-glucopyranoside (1), 2-(3-hydroxy-4-methoxyphenyl)-ethyl-O-beta-D-glucopyranosyl (1-->3) beta-D-glucopyranoside (2), scroside B (3), hemiphroside A (4), plantainoside D (5) and scroside A (6), respectively.</p><p><b>CONCLUSION</b>Compounds 1, 2, 4 and 5 were isolated from this plant for the first time and compound 2 was firstly obtained from natural source.</p>
Subject(s)
Disaccharides , Chemistry , Glucosides , Chemistry , Molecular Conformation , Molecular Structure , Picrorhiza , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Bombyx batryticatus.</p><p><b>METHOD</b>The chemical constituents were isolated from the title materials by column chromatography using silica gel, purified by crystallization, and identified by spectroscopic methods.</p><p><b>RESULT</b>Seven compounds were isolated and identified.</p><p><b>CONCLUSION</b>Six compounds were isolated from Bombyx batryticatus for the first time.</p>
Subject(s)
Animals , Bombyx , Chemistry , Mannitol , Chemistry , Materia Medica , Chemistry , Palmitic Acid , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from the roots of Polygala tenuifolia.</p><p><b>METHOD</b>Column chromatographic techniques were employed for isolation and purification of chemical constituents of the plant and the structures were elucidated by spectroscopic analysis.</p><p><b>RESULT</b>Five chemical constituents were isolated and elucidated as 4-C-beta-glucopyranosyl-1,3,6-trihydroxy-7-methoxyxanthone (1), 4-C-[beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranosyl]-1,3,6- trihydroxy-7-methoxyxanthone (2), presenegenin (3), presenegenin-3-O-beta-D-glycopyranoside (4) and daucosterol (5), respectively.</p><p><b>CONCLUSION</b>Compounds 1,3,4 and 5 were isolated from this plant for the first time. Compound 1 is a new natural product.</p>