Photothermal therapy against breast cancer 4T1 cells using PEGylated gold triangular nanoparticles / 医学研究生学报

Objective To observe the photothermal therapeutic effect of PEGylated gold triangular nanoparticles (PEG-GTN) to breast cancer 4T1 cells upon 606-nm laser irradiation. Methods The obtain gold triangular nanoparticles were functionalized with SH-PEG and incubated with 4T1 cells. The cellular toxicity and photothermal therapeutic effect of PEG-GTN to 4T1 cells were measured by MTT assays. Results After incubated with 5, 10. 20 and 40 µg/mL PEG-GTN for 24 h, the cellular viability were (96.2±4.4)%,(95.9±4.4)%,(95.2±4.8)% and (96.6±4.7)%. There were no statistical differences between PEG-GTN treated groups and control group. With the irradiation of 660-nm laser irradiation, the cellular viability of 4T1 cells were (92.2±6.2)%,(51.6±6.8)%,(25.7±4.5)% and (4.8±2.5)%. The cells treated with PEG-GTN with concentrations equal or higher than 10 µg/mL showed significant lowered cell viability. Conclusion PEG-GTN has low cytotoxicity and concentration-dependent photothermal effect.

CT diagnosis and differential diagnosis of renal cell carcinoma associated with Xp11.2 translocation/TFE gene fusions / 医学研究生学报

Objective XPll.2/TFE RCC is an independent subtype of renal cell carcinoma, which is rare. The aim of this study is to investigate the CT features of Xp11.2/TFE RCC and improve the accuracy of diagnosis. MethodsCT findings of 30 cases from August 2009 to September 2017 of Xp11.2/TFE RCC from Eastern War Zone General Hospital, including location, density, edge, enhancement degree, lymphatic metastasis and others. They were analyzed respectively and compared with those of ccRCC. ResultsThe differences in CT values between tumors and renal cortex and renal medulla was statistically significant different phases (PP< 0.01). Most of patients with Xp11.2/TFE RCC are younger, about (36.4±17.7) years old. Females are more common, accounting for about 70% compared with ccRCC(50%). CT plan scan showed slightly higher density, about (45.2±8.9)HU vs (34.1±4.4)HU, high calcification rate, about 46.7% % vs 10.0% and CT scan with contrast agent showed gradual enhancement. The difference all were statistically significant(P<0.05). Conclusion XPll.2/ has certain CT characteristics. Combined with CT and clinical manifestation of patients, it is helpful to improve the accuracy of preoperative diagnosis.