ABSTRACT
Objective Through statistical analysis of drug resistance and homology of Acinetobacter baumannii in geriatric department of our hospital,we can provide evidence for clinical experience in antibiotics use and nosocomial infection control.Methods The drug resistance of Acinetobacter baumannii isolated from the elderly rehabilitation department in Ningbo Kangning Hospital from January 2017 to December 2018 was analyzed by retrospective analysis. The homology of Acinetobacter baumannii isolated from clinical isolates was detected by PCR ERIC －PCR method and agarose gel electrophoresis method.Results The resistance rates of Acinetobacter baumannii to ciprofloxacin and levofloxacin were 41.6%and 35.4%,respectively.The resistance rates to cefepime ,ceftriaxone and ceftazidime were 40.3%,38.9%and 36.7%,respectively.The resistance rate to imipenem was 38.9%,and the sensitivity rates to cefoperazone／sulbactam,gentamicin and tobramycin were all over 70%.A total of 226 strains of Acinetobacter baumannii were genotyped by ERIC －PCR.They were mainly classified into A,B,C,D and E 5 types.Among them, 116 strains were type A,68 strains were type B,26 strains were type C,8 strains were type D and E.Conclusion The resistance rate of Acinetobacter baumannii to some antibiotics in geriatric rehabilitation department is still at a high level,so we should continue to strengthen the rational use of antibiotics ;at the same time,the strains are highly homologous,and there is likely to be clonal transmission in the department ,so we should take more effective measures to prevent and control its transmission in hospital.
ABSTRACT
Objective@#Through statistical analysis of drug resistance and homology of Acinetobacter baumannii in geriatric department of our hospital, we can provide evidence for clinical experience in antibiotics use and nosocomial infection control.@*Methods@#The drug resistance of Acinetobacter baumannii isolated from the elderly rehabilitation department in Ningbo Kangning Hospital from January 2017 to December 2018 was analyzed by retrospective analysis.The homology of Acinetobacter baumannii isolated from clinical isolates was detected by PCR ERIC-PCR method and agarose gel electrophoresis method.@*Results@#The resistance rates of Acinetobacter baumannii to ciprofloxacin and levofloxacin were 41.6% and 35.4%, respectively.The resistance rates to cefepime, ceftriaxone and ceftazidime were 40.3%, 38.9% and 36.7%, respectively.The resistance rate to imipenem was 38.9%, and the sensitivity rates to cefoperazone/sulbactam, gentamicin and tobramycin were all over 70%.A total of 226 strains of Acinetobacter baumannii were genotyped by ERIC-PCR.They were mainly classified into A, B, C, D and E 5 types.Among them, 116 strains were type A, 68 strains were type B, 26 strains were type C, 8 strains were type D and E.@*Conclusion@#The resistance rate of Acinetobacter baumannii to some antibiotics in geriatric rehabilitation department is still at a high level, so we should continue to strengthen the rational use of antibiotics; at the same time, the strains are highly homologous, and there is likely to be clonal transmission in the department, so we should take more effective measures to prevent and control its transmission in hospital.
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[ ABSTRACT] AIM:To study the effect of livin gene-modified bone marrow mesenchymal stem cells ( BM-MSCs) transplantation on the cardiac function following acute myocardial infarction in a rat model and the expression of livin , caspase-3, caspase-7 and caspase-9 in the livin gene-modified BM-MSCs.METHODS: The MSCs were obtained by the whole bone marrow culture method , and the apoptosis of the MSCs after infection with adenovirus vector carrying enhanced green fluorescent protein ( EGFP) gene and livin recombinant vector ( rAd-livin) were detected by flow cytometry .The ex-pression of livin, caspase-3, caspase-7 and caspase-9 was detected by Western blot .After permanent left anterior descend-ing artery occlusion , the rats were randomized to receive intramyocardial injection of DMEM without cells ( vehicle group ) , or containing MSCs ( MSCs group ) , MSCs ( EGFP ) ( rAd-control/MSCs group ) or MSCs ( livin ) ( rAd-livin/MSCs group).Left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), the maximum in-creased rate of left ventricular pressure ( -dp/dtmax ) and the maximum decline rate of left ventricular pressure ( +dp/dtmax ) were recorded for evaluating the cardiac functions .RESULTS: The apoptosis of rAd-livin/MSCs was significantly decreased as compared with MSCs and rAd-control/MSCs (P<0.05).Meanwhile, the expression of caspase-3, caspase-7 and caspase-9 was significantly downregulated as compared with the other 2 groups ( P<0.05 ) .The cardiac function in rAd-livin/MSCs group was significantly improved as compared with DMEM group , and those in the other 2 groups got the similar results, but the function in rAd-livin/MSCs group was better improved .Meanwhile, the number of surviving cells in rAd-livin/MSCs group was significantly improved as compared with the other 2 groups .CONCLUSION:The apoptosis of MSCs is decreased after rAd-livin transfection, and the expression of caspase-3, caspase-7 and caspase-9 is also significant-ly downregulated while the expression of livin is significantly upregulated .Transplantation of livin-modified BM-MSCs by lentiviral vector results in better prognosis for treating myocardial infarction by enhancing cell survival .