ABSTRACT
Among different bacterial cultures, a potent Bacillus subtilis MTCC-8114 was isolated from garden soil samples which showed 16 and 14 mm inhibition zones by spot inoculation method and 24 and 22 mm inhibition zones by well agar diffusion method against test fungi i.e. Microsporum fulvum and Trichophyton species. Among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (TSB) medium at 37 degrees C, pH-7 and 48 h of incubation. The Rf value (0.64) by Thin Layer Chromatography (TLC) technique and UV and FTIR spectral data of the active antifungal compound, indicated that the isolated compound belongs to peptide antifungal antibiotic group. MIC value of antifungal antibiotic was 135 and 145 microg/ml.
Subject(s)
Antifungal Agents/biosynthesis , Antifungal Agents/pharmacology , Bacillus subtilis/drug effects , Bacillus subtilis/growth & development , Bacillus subtilis/metabolism , Culture Media , Microbial Sensitivity Tests , Microsporum/drug effects , Peptide Biosynthesis/drug effects , Pest Control, Biological , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , Trichophyton/drug effectsABSTRACT
Prevalence of keratinophilic fungi was observed in paddy field soil during different stages of cultivation viz., transplanting, tillering, milking and maturation. Out of total 76 soil samples, 65 soil samples were found to be positive for the keratinophilic fungi. Fourteen species belonging to a single genus Chrysosporium were isolated through out the cropping season. C. keratinophilum (17.1%) followed by C. tropicum (13.15%) were found to be the most dominating geophilic species. The highest percent distribution of keratinophilic fungi appeared during the milking stage (100%) of paddy cultivation, followed by the maturation stage (89.47%).
Subject(s)
Arthrodermataceae/classification , Crops, Agricultural/growth & development , Fungi/classification , Keratins/metabolism , Oryza/growth & development , Seasons , Soil Microbiology , Time Factors , WetlandsABSTRACT
Aspergillus was found as a dominant fungi to associate with brands of bindis. Among three potencies of four homeopathic drugs, Lycopodium 1M, Sulphur 1M, and Sepia 30 showed maximum inhibition zone of Aspergillus niger in inhibition zone technique. In poison food technique, Sepia 30M, Tellurium 30M, Sulphur 1M and Lycopodium 200 showed maximum percentage inhibition against A. niger
Subject(s)
Adhesives , Animals , Antifungal Agents/pharmacology , Aspergillus niger/drug effects , Cosmetics , Dermatitis, Allergic Contact/microbiology , Homeopathy , Lycopodium/chemistry , Microbial Sensitivity Tests , Sepia/chemistry , Sulfur/pharmacology , Tellurium/pharmacologyABSTRACT
A potent Bacillus subtilis-2 isolated from soil samples, showed 14 mm inhibition zone by spot inoculation method and 22 mm inhibition zone by well agar diffusion method against pathogenic test fungii. i.e. Trichophyton species. Among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (TSB) medium at 37 degrees C, pH 7 and 48 hrs of incubation. The MIC of antifungal substance was 35 microl/ml. The isolated Bacillus subtilis -2 has strong antifungal activity against dermatophytic fungi Trichophyton sp. and requires chemical characterization for its bioactive principle.