ABSTRACT
Microspheres (MS) are an excellent transarterial chemoembolization carrier for cancer treatment. Then the Bletilla striata polysaccharide (BSP) that was isolated from the rattan of Bletilla striata was used as skeleton material, and the matrine (ME) loaded Bletilla striata polysaccharide microspheres (ME-BSPMS) were prepared by emulsify-chemical crosslinking method. ME-BSPMS was characterized for appearance shape, particle size, drug loading, swelling ratio, suspension property, drug entrapment condition and in vitro release characteristics. The results showed that the ME-BSPMS appeared as round spherical and smooth shape by SEM, with an average size of (85 ±7) μm. ME-BSPMS with a good suspension in physiological saline and the swelling ratio could reach upwards of (53 ±4.2)% in 20 minutes, also with a large amount of drug loading of (30.12 ±3.25)%. The results of DSC scanning indicate that good compatibility exists between the ME and BSP, and the ME could be embedded fully in the matrix of the ME-BSPMS. The accumulation drug release from ME-BSPMS was (25.38 ±1.57)% at 12 h, this suggests that the ME-BSPMS has a good sustained release effect. These results indicate that the ME-BSPMS may be a promising transarterial chemoembolization carrier for cancer treatment.
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Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3%HAc-Methonal(74∶26). The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL/min. Separation column was Kroasil ODS(4.6 mm?250 mm). Result A good linearity was obtained in the range of 65～325 ?g,regression A=24 239 869C-31 292,r=0.999 3. The average recovery was 100.10%,RSD was 0.87%(n=6). Conclusion The method was good for determining chlorogenic acid in Jinyin Granules.
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Objective To investigate the action and the mechanism of Jinyin granule(JYG) on cholecystitis in guinea pigs.Methods Cholecystitis models of Guinea pigs were established and then randomly divided into six groups: normal group,model group,XiaoYanLiDan tablet(XYLDT) group,treatment groups including low,middle and high dosage groups of JYG.The Pigs were given above drugs respectively while in normal and model groups the same volume of normal saline was given.Body weight,gallbladder weight,bile amount,IL-1? in the serum were measured and gallbladder pathological tissue was observed by light microscopy.Results Comparing with the model group,the treatment groups had higher body weight,lower gallbladder weight,less bile amount,obviously slighter inflammation and less amount of IL-1? in the serum.Conclusion JYG possesses the therapeutic action to cholecystitis on guinea pigs and its mechanism is interrelated to the decreasing of IL-1? in the serum.
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OBJECTIVE:To study the effect of Erdong granules on glucose metabolism and lipid metabolism in type 2 diabetes mellitus rats.METHODS:Type 2 diabetes mellitus model was induced by giving high-fat and high-calorie diet with intraperitoneal administration of streptozotcin for eight weeks.Model rats were divided into normal group,model group,streptozotcin group and erdong granules high-dose,middle-dose and low-dose groups.The levels of FBG,LDL-C,FFA,SOD and MDA were detected and immunohistochemistry method was used to determine the morphology change of islet cell.RESULTS:The serum levels of FBS,MDA,FFA and LDL-C in Erdong granules high-dose and low-dose group were significantly decreased while the activity of SOD was increased.Erdong granules could protect islet cell.CONCLUSION:Erdong granules can notably improve glucose metabolism and lipid metabolism,antioxidant enzyme activity and inhibit oxidative stress so as to protect islet cells of type 2 diabetic mellitus rats.
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Objective To find a new therapy for chronic bacillary prostatitis (CBP) with intervention. Methods According to Seldinger way, the catheter was inserted into internal iliac artery or inferior vesical artery percutaneously and was perfused medicine. Results Of which 10 cases recovered and 2 increasingly improved. And there was no positive prostate bacterial culture. But after 3 to 6 month survey, one patient relapse. Conclusion The arterial-cathertered perfusion is a safe, simple and low recurrence way for CBP and available to further study.
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14 strains were isolated from pits sludge by anaerobic cultivation,two Clostridium butyricum strains were identified by physiological and biochemical experiments and the sequence analysis of 16S rDNA.The physiological characteristics and security of Clostridium butyricum B1 were studied,in vitro research indicated it was tolerant against low pH,bile concentration and antibiotics and has antagonism effects against pathogens.