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Journal of Forensic Medicine ; (6): 373-380, 2015.
Article in Chinese | WPRIM | ID: wpr-984016

ABSTRACT

OBJECTIVE@#To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.@*METHODS@#Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.@*RESULTS@#A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.@*CONCLUSION@#The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.


Subject(s)
Humans , Amelogenin , Chromosomes, Human, Y/genetics , DNA Primers , Databases, Nucleic Acid , Forensic Genetics/methods , Genotype , Genotyping Techniques/instrumentation , Indicators and Reagents , Microsatellite Repeats , Multiplex Polymerase Chain Reaction
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