Screening for specific membrane protein of Schistosoma japonicum and cloning of sequence encoding protein sj15 / 第二军医大学学报

Objective: To screen for the potential specific membrane protein from the protein database built by our Lab and to clone the encoding sequence of a potential immunoprotective protein according to the screening result, so as to search for new vaccine candidate or drug targets for Schistosomiasis control. Methods: The related data were retrieved using the key words "Schistosoma japonicum" and "membrane or tegument" from the NCBI database to construct local database of membrane protein of S. japonicum. The specific membrane proteins were screened by multi-alignment with the protein sequences from our local database of Schistosoma mansoni, Homo sapiens, Rattus norvegicus and Mus musculus. Meanwhile, the character and function of the predicted membrane protein were analyzed with public software in the website CBS. The specific primer for the sequence of the predicted protein with potential immuno-protective function were designed and synthetized; the coding sequence was then amplified from the adult worm of S. japonicum by PCIZ and was cloned into rokaryotic expression vector pGEX-4T-1. Results: A total of 153 predicted membrane protein of S. japonicum were retrieved and 28 potentially specific for S. japonicum were screened out. The coding sequence of the predicted protein, with a relative molecular weight of about 15 000, was successfully amplified from the adult worm of S. japonicum and was temporarily named gene sj15; the recombinant plasmid pGEX-4T-1-sjl5 was also successfully constructed. Conclusion: Many specific membrane proteins of S. japonicum have been successfully screened out. The predicted sjl5 gene (announced as AY223457 in GenBank) has been successfully cloned, proving the existence of sj15 gene in adult worm of S. japonicum. The prokaryotic expression vector pGEX-4T-1 for sj15 has also been successfully constructed, providing a foundation for further study of sj15 function.

Case-control study of risk factors in cholangiocarcinoma / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the risk factors of intrahepatic cholangiocarcinoma (ICC) and extrahepatic cholangiocarcinoma (ECC).</p><p><b>METHODS</b>The clinicopathological data of 190 patients with cholangiocarcinomas (61 ICC and 129 ECC) diagnosed and treated in the Peking Union Medical College Hospital between 1998 and 2008 were collected. The clinicopathological data of 380 matched healthy controls were also collected. The information about liver diseases, family history, diabetes, smoking and drinking were recorded and analyzed.</p><p><b>RESULTS</b>The positive rate of HBsAg(+) and anti-HBc(+), HBsAg(-) and anti-HBc(+) and the incidence of choledocholithiasis or hepatolithiasis in ICC patients were 27.9%, 50.8% and 14.8%, respectively. The incidence of diabetes mellitus, cholecystolithiasis, choledocholithiasis or hepatolithiasis and previous cholecystectomy in ECC patients were 18.6%, 15.5%, 18.6% and 13.2%, respectively. The incidences of all above mentioned factors in the ICC or ECC patients were significantly higher than that in the controls (P < 0.05). Compared with the patients with ECC, the ICC patients had a significantly higher cirrhosis rate (P < 0.05).</p><p><b>CONCLUSION</b>Our study results show that choledocholithiasis or hepatolithiasis, liver cirrhosis and chronic HBV infection are possible risk factors for intrahepatic cholangiocarcinoma, while choledocholithiasis or hepatolithiasis, diabetes mellitus, cholecystolithiasis, history of cholecystectomy are risk factors for extrahepatic cholangiocarcinoma.</p>

Clinical value of "Kou mode of hepatic hilar anastomosis" in resection of type III or IV hepatic hilar cholangiocarcinoma / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To evaluate the surgical technique of "Kou mode of hepatic hilar anastomosis" in the treatment for type III or IV hilar cholangiocarcinoma.</p><p><b>METHODS</b>The clinical data of 89 patients with type III or IV hilar cholangiocarcinoma surgically treated in our department between Jan. 1990 and Jan. 2008 were retrospectively analyzed. Since January 2000, "Kou mode of hepatic hilar anastomosis" was performed for some patients with advanced hilar cholangiocarcinoma. The patients were divided into two groups: group A treated between 1990 and 1999, group B between 2000 and 2008. The rate of resection, therapeutic efficacy and complications in these two groups were compared, respectively.</p><p><b>RESULTS</b>Of the 37 cases with hilar cholangiocarcinoma in group A, 4 were surgically treated (10.8%), with 1 (2.7%) radical resection and 3 (8.1%) palliative resection. Among the 52 cases with hilar cholangiocarcinoma in the group B, 35 (67.3%) received surgical resection, of them 15 (28.8%) underwent radical resection and 20 (38.5%) had palliative resection. Twenty-eight of these 35 cases underwent the "Kou mode of hepatic hilar anastomosis". The resection rate of advanced hilar cholangiocarcinoma in the group B was significantly higher than that in group A (P < 0.05). The complications in the 89 cases included ascites (3 cases), hemobilia (1 case), heart failure (1 case), and wound infection (2 cases). All the patients who were treated with the "Kou mode of hepatic hilar anastomosis" developed bile leakage to a varying degree and recovered after drainage and symptomatic treatment.</p><p><b>CONCLUSION</b>The resection rate of type III or IV advanced hilar cholangiocarcinoma can be remarkably improved by using a novel alternative surgical technique called "Kou mode of hepatic hilar anastomosis". However, the long-term outcome still needs to be determined by close follow-up and further observation.</p>

Results of multimodality therapy for unresectable primary liver cancer / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the therapeutic measures for unresectable primary liver cancer (PLC).</p><p><b>METHODS</b>The date of 312 unresectable primary liver cancer patients treated from January 1991 to March 2003 were retrospectively analyzed.</p><p><b>RESULTS</b>Of these 312 patients, 73 were treated by cryosurgery-based combined modality therapy, 239 were treated by a TACE-oriented combined modality therapy. 289 patients except 23 were followed for a period of 2 to 156 months. The overall 1-,3- and 5-year survival rate in this series was 74. 0% , 34. 0% and 16. 7% , respectively. The 1-,3-and 5-year survival rate in the cryosurgery group was 64. 4% , 38. 4% and 27. 4% , respectively. The 1-, 3- and 5-year survival rate in the TACE group was 75. 1% , 29. 0% and 10. 0%, respectively.</p><p><b>CONCLUSION</b>Treatment for the unresectable primary liver cancer should be individualized and combined with suitable therapeutic modalities.</p>

Treatment of prolapsed hemorrhoids with circular stapler / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of circumferential mucosectomy procedure for treatment of prolapsed hemorrhoids (PPH).</p><p><b>METHODS</b>From June 2001 to June 2003, 74 patients (27 men and 47 women) with an average age of 57 years (ranging from 31 to 80 years), with prolapsed hemorrhoids III - IV degree underwent PPH using a circular stapler.</p><p><b>RESULTS</b>69 (93.2%) patients were fully satisfied with results. Two patients underwent simultaneous rectal polypectomy along with PPH hence required analgesic treatment for 5 days. Three patients experienced bleeding during or after operation, 1 case bleeding was due to ulcerative hemorrhoid, while the bleeding the remaining 2 cases was (bleeding about 300 ml) caused by insufficient anastomosis, thus extending operating time to 1 hour. The average operation time (70 patients) was 13 minutes (range 10 - 15 minutes). The mean hospitalization was 3.5 days (2 - 4 days), with exception of 2 patients lasting 1 week.</p><p><b>CONCLUSION</b>PPH is a safe, effective and rapid method for treatment of prolapsed hemorrhoids, The procedure causes minimal pain with decreased complications.</p>