ABSTRACT
<p><b>UNLABELLED</b>To set up a new rapid and efficient way for the preparation of specific monoclonal antibodies (MAbs) with plasmid DNA immunization.</p><p><b>METHODS</b>The fusion gene of IL-2 signal peptide and profilin 1 by 'overlapping PCR' was obtained and inserted into the vector pVAX1 to construct the recombinant plasmid pVAX-IL2-profl. Another fusion gene of IgG kappa chain signal peptide and profilin 1 by 'no template PCR' was obtained and inserted into the vector pVAX1 to construct the recombinant plasmid pVAX-Igkappa-prof1. BALB/c mice were single intrasplenic immunized with plasmid DNA. Results After cell fusion and hybridomas screening by indirect ELISA, we charactered two mAbs (1D8A2B4 and 4B12A5E3) against profilin 1. The MAbs isotype were determined as IgM and IgG3.</p><p><b>CONCLUSION</b>A single intrasplenic plasmid DNA immunization is rapid and efficient and can be used as a helpful tool for the production of specific mAb.</p>
Subject(s)
Animals , Female , Mice , Antibodies, Monoclonal , Genetics , Allergy and Immunology , Antibody Specificity , DNA , Genetics , Allergy and Immunology , Gene Fusion , Immunization , Interleukin-2 , Genetics , Allergy and Immunology , Mice, Inbred BALB C , Plasmids , Genetics , Profilins , Genetics , Allergy and Immunology , Spleen , Allergy and Immunology , MetabolismABSTRACT
Rhamnolipid,an important biosurfactant,is reviewed with respect to chemical strocture,properties,physiological role and their fermentation production,especially focusing on the production with inexpensive raw materials,such as vegetable oils and residues from agro- industrial wastes.This can not only reduce the production costs,but also contribute to the reduction of environmental impact generated by the discard of residues,and the treatment costs.