ABSTRACT
Objective To study the genetic relationship between Kirgiz individuals living in Sinkiang China and analyze the difference among Kirgiz and the other population with STR polymorphisms. Methods PCR amplification was performed using PE9700, the PCR products were typed by automated sequencer and genescan. Results A database of nine STR loci of Kirgiz was established. It shows there are at least 73 STR alleles and 191 genotypes in Kirgiz. Genotype frequencies distribution showed no deviation from Hardy-Weinberg equilibrium by χ2-test. Kirgiz was compared with the other Chinese ethnic groups, then the American Black and the White. Conclusion These results suggested that the nine STR loci and Amelogenin locus were very useful in human identification, biological archaeology and gene resource studies.
ABSTRACT
Objective: To study the genetic relationship between Kirgiz individuals living in Sinkiang China and analyze the difference among Kirgiz and the other population with STR polymorphisms. Methods: PCR amplification was performed using PE9700, the PCR products were typed by automated sequencer and genescan. Results: A database of nine STR loci of Kirgiz was established. It shows there are at least 73 STR alleles and 191 genotypes in Kirgiz. Genotype frequencies distribution showed no deviation from Hardy-Weinberg equilibrium by χ2 -test. Kirgiz was compared with the other Chinese ethnic groups, then the American Black and the White. Conclusion: These results suggested that the nine STR loci and Amelogenin locus were very useful in human identification, biological archaeology and gene resource studies.