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Objective To investigate the relation between course and effect of hyperbaric oxygen therapy on patients with spinal cord injuries. Methods The retrospective study of 147 patients with spinal cord injuries were reviewed and on the basis of comprehensive treatment the control group was not treated with hyperbaric oxygen (NHBO group,39 cases),whereas Hyperbaric oxygen treatment group(HBO group,108 cases)were treated with hyperbaric oxygen therapy. According to courses of HBO treatment ,from short term to long term ,the HBO group was divided into 3 subgroups,HBO1,HBO2 and HBO3 subgroup. Evaluation based on the result all selected patients assessed on the six months after the date of surgery. Results There was no significant difference in baseline data between the NHBO group and the HBO group and the overall effectiveness of the HBO group was better than the NHBO group(P<0.01). The efficacy of HBO2 and HBO3 subgroup was better than that of HBO1 subgroup. From the perspective of spinal cord function before and after treatment ,the efficacy of the HBO group was better than that of the NHBO group(P < 0.05).The efficacy of HBO2 and HBO3 subgroup was superior to that of HBO1 subgroup (P < 0.05). However there was no significant difference in total effective rate and spinal cord score between the HBO2 and the HBO3 subgroups. Conclusions Hyperbaric oxygen therapy is effective in the treatment of spinal cord injuries. The treatment for 3~4 courses play the best therapeutic effect.
ABSTRACT
Objective To study the effects of hyperbaric oxygen (HBO) preconditioning (PC) on motor function and mitochondrial apoptosis after spinal cord injury (SCI) in rats.Methods Thirty-six healthy,male Wistar rats were randomly divided into 3 groups (each n =12):a control (CON) group,an SCI model group and an HBO-PC group.The SCI group and the HBO-PC group after 7 sessions of HBO-PC treatment were subjected to SCI modeling using Allen's method.The CON group was not given any special treatment.Two weeks after the modeling,Basso,Beattie,and Bresnahan BBB scale was used to rate the rats' locomotor function,and the T8 segment of the rats' spinal cords was removed.After extracting total RNA from the spinal cord tissue,real-time quantitative PCR was used to detect the mRNA expression levels ofcaspase-3,cytochrome C (CYC),Bak,Bax,Bcl-x and Bcl-2 in the spinal cord tissue.Results Two weeks after modeling,compared with the CON group,the average BBB locomotion score of the SCI model group had decreased.In contrast,caspase-3,CYC,Bak and Bax mRNA expressions had increased significantly.Compared with the CON group,the average BBB locomotion score of the HBO-PC group had decreased,caspase-3 expression had increased,and Bax mRNA expression had decreased,but the differences in the changes between the two groups were not statistically significant.Bak mRNA expression increased and CYC expression decreased,and the difference between the two groups was statistically significant.Compared with the SCI model group,the average BBB locomotion score of the HBO-PC group increased while caspase-3,CYC,Bak and Bax mRNA expressions decreased,but none of these differences between the two groups was statistically significant.Bak mRNA expression increased and CYC mRNA expression decreased,and those differences between the two groups were statistically significant.Bcl-x and Bcl-2 mRNA expression in the CON,HBO-PC and SCI model groups were significantly different.Conclusion HBO-PC can reduce the loss of motor function after SCI,at least in rats.The mechanism may be related to reducing neuron mitochondrial apoptosis.