ABSTRACT
Objective:To investigate the protein expression of GTPBP4 in human hepatocelluar carcinoma (HCC) tissues and the influ-ence of GTPBP4 silencing by siRNA on the proliferation and cell cycle of HCC cell line Hep G2. Methods:Western blot analysis was per-formed to observe the protein expression of GTPBP4 in 24 cases of HCC tissues compared with their adjacent noncancerous liver tis-sues. Lentivirus-mediated RNA interference (RNAi) was used to silence GTPBP4 expression in Hep G2, and the infection efficiency was observed under a fluorescence microscope. The silencing effect was tested by Western blot and real-time PCR. After silencing the GT-PBP4 gene, cell proliferation was detected using CCK-8 assay, and the cell cycle was observed using flow cytometry. Results:(1) GT-PBP4 was overexpressed in 21 cases (87.5%) of HCC tissues (P<0.000 1). (2) After the lentivirus with GFP reporter infected the Hep G2 cells, 90%of the cells showed green fluorescence. LV-GTPBP4-RNAi effectively inhibited the expression of GTPBP4 at both mRNA (70%) and protein (67%) levels. (3) The proliferation ability of the LV-GTPBP4-RNAi group significantly decreased after 96 h (inhibition rate:54.51%). Flow cytometry showed that the LV-GTPBP4-RNAi group significantly increased at the G0/G1 phase, whereas the S phase de-creased and arrested at the G0/G1 phase. Conclusion:GTPBP4 overexpression in HCC tissues was associated with hepatocarcinogenesis and promoted tumor cell proliferation, but the specific molecular mechanisms remain to be investigated.
ABSTRACT
Objective To compare and observe the clinical effects between abdominoscope conservative surgical management and laparotomy conservative surgical management for tubal pregnancy.Methods From January 2007 to January 2008,112 cases of tubal pregnancy patients were randomly classified into two groups,which abdominoscope surgery with 67 cases and abdominal surgery with 45 cases.Results The operation time,body temperaturerecovery time and anus start deflation time in abdominoscope surgery group were significantly shorter than those in abdominal surgery group(P ＜ 0.05).The tubal patency rates and 1 year conception rates in abdominoscope surgery group were significantly higher than those in abdominal surgery group(P ＜ 0.05).Conclusion Abdominoscope conservative surgical management treated tubal pregnancy is the best choice for those with breed requirement,which insult is small,bleeding amount is few,length of stay is short and recovery is quick.