Study on HPLC Fingerprint of Bining Nasal Spray / 中国药房

OBJECTIVE:To establish HPLC fingerprint of Bining nasal spray.METHODS:HPLC method was performed.The determination was performed on Neptune C18 column with mobile phase consisted of acetonitrile-0.2 ％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 326 nm,and the column temperature was 25 ℃.The sample size was 10 μL.Using rutin as reference,HPLC chromatograms of 20 batches of samples were determined.Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System for TCM Fingerprint (2012 edition).RESULTS:There were 40 common peaks in HPLC chromatograms of 20 batches of Bining nasal spray,with similarity ＞0.9.After validation,HPLC chromatograms of 20 batches of samples were in good agreement with the control fimgerprints of those.CONCLUSIONS:Established fingerprint can provide reference for identification and quality evaluation of Bining nasal spray.

Study on Material Basis of Miao Medicine Bi-Ning Spray in Allergic Rhinitis Treatment / 世界科学技术-中医药现代化

This paper was aimed to study the material basis for the efficacy of Miao medicine Bi-Ning spray in the treatment of allergic rhinitis,and to lay a foundation for improvement of its quality standard.Solidago and Centipeda minima were weighed according to the prescription and then soaked in water.Reflux extraction was conducted to the liquid.Petroleum ether,ethyl acetate,and n-butanol were used in the extraction,respectively.Each received composition was dried and made into sample solution for the experiment.A total of 60 healthy adult SD rats,with the ratio of half male and half female,were selected and randomly divided into 6 groups according to the weight with 10 rats in each group.There were the normal group,the model group,the petroleum ether (SYM) group,the ethyl acetate (YSYZ) group,the nbutyl alcohol (ZDC) group and the water group.The 20％ xylene olive oil solution was used in the model establishment of rats from different groups except the normal group.Intranasal administration of drug was given to different dosage group after the models were successfully established.Normal saline was given to the normal group and the model group.Serum of each experimental rat was collected at the end of the experiment.The contents of IgE,IL-4 and HT in serum were detected by ELISA.The pathological morphology of the nasal mucosa was analyzed.The results showed that compared with the normal group,the contents of IgE,IL-4 and HT in serum of the model group increased significantly (P ＜ 0.05).Compared to the model group,the contents of IgE,IL-4 and HT in serum of the water group decreased significantly (P ＜0.05).Contents of IgE,IL-4 and HT in other groups presented decreasing tendency.However,there was no statistical significance.The pathological morphology of the nasal mucosa results showed that compared with the normal group,the cell inflammatory response of the nasal mucosa tissues significantly increased in the model group (P ＜ 0.01).Compared with the model group,the cell inflammatory response of nasal mucosa tissues significantly decreased in the ZDC group and water group (P ＜ 0.05).There was no statistical significance in other groups.It was concluded that components in water part were the main material basis for efficacy of Bi-Ning spray in the treatment of allergic rhinitis.

Study on the Anti-influenza A Virus H1N1 Effect of Aqueous Extraction of Lonicerae Flos in vitro / 中国药房

OBJECTIVE:To investigate the effect of aqueous extraction of Lonicerae Flos (SYHW) on anti-influenza A virus H1N1 (H1N1 virus) in vitro. METHODS:Using Madin-Darby canine kid ney (MDCK) cells cultured in vitro by H1N1 virus, half of the tissue culture infection dose(TCID50)was calculated. Culturing MDCK cells for 24 h with different mass concentrations of SYHW,the maximum non-toxic concentration was investigated. And then test was divided into normal cell group,virus control group,SYHW preventive administration group,therapeutic administration group and direct killing group (given SYHW of maxi-mum non-toxic concentration,infecting cells by 100 TCID50 H1N1 virus),and antiviral effective rate (ER) of SYHW was deter-mined. Test was divided into normal cell group,virus control group,SYHW therapeutic group and direct killing group (the same administration and infection as above),changes of cell proliferation index (PI) and cell apoptosis rate were respectively deter-mined. RESULTS:100 TCID50 of H1N1 virus was 1.26×10-7,and the maximum non-toxic concentration of SYHW on MDCK cells was 50 μg/mL(cell survival rate was 91.3%). ERs of preventive administration group,therapeutic administration group and direct killing group were 0,80.3% and 52.7%,respectively. Compared with normal cell group,PI value in virus control group was sig-nificantly reduced (P<0.05),early and late apoptotic rates were significantly increased (P<0.05). Compared with virus control group,PI value in directly killing group was significantly increased(P<0.05),and early apoptotic rate was significantly reduced (P<0.05);early apoptotic rates in therapeutic administration group were significantly reduced (P<0.05). CONCLUSIONS:SY-HW shows anti-H1N1 virus effect in vitro,therapeutic administration and directly killing are preferred in antiviral effect.

The protection effect and mechanism of aloe polysaccharide on experimental colitis mucosa / 实用医学杂志

Objective To investigate the protective effect of aloe polysaccharide on experimental colitis mucosal. Methods The TNBS-induced experimental rat colitis model was successfully established and were treated by aloe polysaccharide, and enteric-coated tablets with sulfasalazine (SASP). Results DAI score, gross morphology score , histopathologic score and MPO in the model group were significantly higher than that in the normal group (P < 0.01), while those indicators were significantly lower after treatment (P < 0.01), IL-12 and IFN-γ mRNA and protein expressions in the model group were significantly higher than that in the normal group (P < 0.01), and IL-12 and IFN-γ expression levels in treatment group were significantly lower than that in the model group (P < 0.01), IL-4 and IL-13 mRNA and protein expression levels in the model group were significantly lower than that in the normal group (P < 0.01), and IL-4 and IL-13 expression in treatment group was significantly higher than that in the model group (P < 0.01). Conclusion Aloe polysaccharide on experimental colitis in rats has a good protective effect , of which mechanism may be reduced by IFN-γ and IL-12 levels, increased IL-4, IL-13 levels, and correct the imbalance of Thl/Th2.

Dermatopharmacokinetic studies of liangfu cream in mice skin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the relationship between deposition content and time of the active ingredients in rat skin, and investigate the dermatopharmacokinetics of Liangfu Cream.</p><p><b>METHOD</b>The contents of paeonol, dictamnine, fraxinellone and glycyrrhetinic acid in rat skin were determined by HPLC. The dermatopharmacokinetics parameters were calculated by DAS software.</p><p><b>RESULT</b>The dermatopharmacokinetics of paeonol and glycyrrhetinic acid were two compartment model, while that of dictamnine and fraxinellone were one compartment model: T(1/2Ka) of four active ingredients were 0.307, 0.112, 0.146, 0.216 h, respectively; T(lag) of them were 0.006, 0.123, 0.136, 0.109 h, respectively; all the Tmax of them was 0.5 h; the Cmax, were 40.163, 1.607, 6.725, 100.553 microg x cm(-3), respectively; the t(1/2beta), were 14.719, 1.262, 0.838, 234.807 h, respectively; the AUC(0-infinity), were 16.987, 2.713, 9.345, 697.000 microg x cm(-3) x h(-1), respectively; and the MRT(0-infinity) were 3.662, 1.67, 1.585, 10.897, respectively.</p><p><b>CONCLUSION</b>The skin pharmacokinetics characteristic of four ingredients in Liangfu cream is lined with the cataplasm long time.</p>

Effects of penetration enhancers on in vitro percutaneous absorption and amount retained in skin of paeonol, dictamnine, fraxinellone and glycyrrhetinic acid in Liangfu cream / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of different penetration enhancers on the in vitro percutaneous absorption and amount retained in skin of active ingredients in Liangfu cream and to screen out the effective accelerator.</p><p><b>METHOD</b>Using improved Franz-type difusion cell and excised small mouse skin in vitro as transdermal barrier, the amount retained in skin and kinetics parameters of active ingredients such as cumulative permeation quantity, permeation rate and permeation lagged time were determined by HPLC. The enhancement ability of four different enhancers such as azone, oleic acid, transcutol P and isopropyl myristate were investigated.</p><p><b>RESULT</b>3% IPM enhanced the cumulative permeation quantity better than other penetration enhancers. The enhancive permeation multiples of paeonol, dictamnine, fraxinellone and glycyrrhetinic acid were 1.52, 1.24, 1.73 and 3.21 times (P < 0.05). The enhancive amount retained in skin multiple of glycyrrhetinic acid was 1.96 times (P < 0.05), but for other components there were no significant impacts.</p><p><b>CONCLUSION</b>The effects of penetration enhancers on the in vitro percutaneous absorption and amount retained in skin of components in Liangfu cream are different. 3% IPM which can enhance the cumulative permeation quantity of four components and amount retained in skin of glycyrrhetinic acid is the most suitable penetration enhancer for Liangfu cream.</p>

Preparation and phase solubility of inclusion complex of cholic acid-hydroxypropyl-?-cyclodextrin / 中成药

AIM: To optimize the preparation of cholic acid-hydroxypropy-?-cyclodextrin inclusion complex,and its phase solubility analysis. METHODS: Orthogonal test,including molar ratio,inclusion temperature,mixing time and liquid dropping rate of cholic acid-HP-?-CD,was adopted to screen the optimal preparation,based on the inclusion efficiency by HPLC method.The solubilization effect was evaluated by using phase solubility.(RESULTS): The optimal preparation consisted of the molar ratio of cholic acid-HP-?-CD was 1∶3,60 ℃ inclusion temperature,60 min mixing time,1.6 mL/min liquid dropping rate;A_L type of phase solubility curve,K=564.30 L/mol,and 11.81 times solubilization. CONCLUSION: The optimal preparation is stable,reasonable and practicable with the encapsulation efficiency of 97.1%.Inclusing cholic acid with HP-?-CD is feasible,and the solubilization effect is significant.