ABSTRACT
Objective:To explore the influencing factors of satisfaction with thyroid nodule fine needle biopsy (FNA).Methods:The ultrasonic images of 88 patients with thyroid nodules (95 nodules) in Dongguan Binhai Bay Central Hospital from September 2016 to January 2019 were analyzed retrospectively. According to Bethesda cytological classification standard, Bethesda class Ⅰ cytological specimens were defined as unsatisfactory, and Bethesda class Ⅱ-Ⅵ cytological specimens were defined as satisfactory. The thyroid nodule location, composition, internal echo, morphology, aspect ratio, calcification, blood supply mode and American College of Radiology Thyroid Imaging Reporting and Data System(ACR TI-RADS) classification on the satisfaction rate of thyroid nodule FNA samples were evaluated, and then the meaningful influencing factors were analyzed by logistic regression.Results:The overall satisfaction of the sample in this study was 84.2% (80/95). The results of univariate analysis showed that the location, aspect ratio and ACR TI-RADS classification of thyroid nodules were the related factors affecting the satisfaction of FNA specimens of thyroid nodules, with statistically significant difference ( P<0.05). Logistic regression analysis showed that the aspect ratio of thyroid nodules was an independent factor affecting the satisfaction of FNA specimens of thyroid nodules ( OR=13.5, P=0.02). Conclusions:The main factor affecting the satisfaction rate of FNA specimens of thyroid nodules may lie in the characteristics of the aspect ratio of the nodules themselves. Optimizing the puncture route may help to reduce the dissatisfaction rate of FNA specimens of thyroid nodules.
ABSTRACT
Objective To observe the changes in expressions of spleen regulatory T cells (Tregs)and the related factor forkhead box protein-3 (Foxp3) after irradiation with different doses of X-ray in mice at different times,and to elaborate the effects of X-rays on regulatory T cells and Foxp3.Methods 112male ICR mice were randomly divided into 2 groups and irradiated by X-rays at the doses of 0.075 and 2 Gy,respectively.The mice were killed at 0,4,8,16,24,48,and 72 h post-irradiation and the spleens removed.Flow cytometry was used to detect the percentage of CD4 + CD25 + Treg and protein expression of (Foxp3),and RT-PCR was used to exmiamine the mRNA expression of Fox3.Results Compared with those before irradiation,the CD4 + CD25 + Treg positive rates began to increase and peaked at 8 h post-irradiation with 0.075 Gy at 8,16,24,72 h(t = 8.73,10.55,4.21,4.65 ,P ＜ 0.05) and 2 Gy at 8,16,48,72 h(t = 4.65,4.28,3.71,2.88,P ＜ 0.05),and then slightly decreased,but still remained at high levels.The mRNA protein levels of Fox3 did not change significantly after exposure to the dose of 0.075 Gy,but began to significantly increase at 8 h after exposure to the dose of 2 Gy.However,the Foxp3 protein level began to increase 4 h post-irradiation,peaked at 16 h,and then slightly decreased,but still ramained at high levels (t =2.59,3.37,3.70,3.20,P＜0.05).Conclusions The changes in expressions of Tregs and Foxp3 after high- and low-dose X-ray irradiation may be used to explain the differences in immune effects induced by ionizing radiation at different doses.