ABSTRACT
Long non-coding RNA urothelial carcinoma associated antigen 1 (UCA1) is highly expressed in multiple types of tumors,such as bladder cancer,breast cancer,colorectal cancer and so on.Recent studies show that UCA1 can interact with the microRNAs as a competing endogenous RNA (ceRNA),to indirectly regulate the expression of target genes,and then participates in the regulation of the initiation and progression of tumor.Therefore,using the mechanism of ceRNA to intervene the expression of UCA1 will provide new ideas for tumor treatment.
ABSTRACT
Objective\ To observe the expression of coreprotein gene in human lumbar discs in foetus and adult. Methods\ The coreprotein cDNA probe was prepared by RT-PCR. The mRNA level and distribution of coreprotein was observed in fetal and adult lumbar discs, using in situ hybridization method. Results\ 1) The highest expression lies in the lumbar nucleus pulposus of the fetal discs, less higher expression in the interior of the annulus fibrosus and the region between nucleus and annulus fibroses. 2) In adult disc tissue, few positive signals were shown. Low gene expression level of coreprotein in the subcultured disc cells was expressed. Conclusion\ The higher expression of coreprotein in fetal discs is chiefly located in the nucleus pulposus.