ABSTRACT
Objective To analysis on the 25-hydroxyvitamin D[25-(OH)D] level of preschoolers in Yangzhou ,evaluating their nutrition conditions ,to discuss the influence factors .Methods The 25-(OH) D in plasma of peripheral vein was measured in the preschoolers who came to Maternal and Child Care Service Hospital of Yangzhou receiving healthy examinations in 2014 all over the year ,SPSS17 .0 software was used to analyze the data .Results The vitamin D level of preschoolers had statistical significance be-tween different age group .Season and vitamin D level of pregnant women also contributed to the statistical significance .Conclusion The vitamin D level of preschooler children shows low ,the supplement of vitamin D of pregnant women could improve the new -borns ,reduce the rate of illness .
ABSTRACT
Objective To investigate the regulation of swimming motility by H-NS in Vibrio parahaemolyticus(VP). Methods VP was inoculated into the semi-solid swimming agar plate containing 1% Oxoid tryptone, 2% NaCl, 0.5%Difco Noble Agar, and 0.1% arabinose followed by incubation at 37℃ for 4.5 h before the diameters of bacterial lawns were measured.Total RNAs were extracted from the wild-type (WT) strains and the hns null mutant (Δhns), and the quantitative real-time( RT)-PCR( qRT-PCR) was carried out to calculate the transcriptional variation of flaA between WT andΔhns strains.The entire promoter DNA region of flaA was amplified and cloned into the lacZ fusion vector pHRP309 containing a promoterless lacZ gene. The recombinant lacZ reporter plasmid was transformed into WT and Δhns, respectively, to measure the β-galactosidase activities in cellular extracts using the β-galactosidase enzyme assay system. Results and Conclusion The phenotype results showed that swimming motility of VP was enhanced by H-NS.The qRT-PCR and LacZ fusion results indicated that the transcription of flaA was positively regulated by H-NS.Collectively, H-NS promotes the swimming motility of VP, at least partly, by activating the transcription of flaA.