ABSTRACT
BACKGROUND/AIMS: This study tested the hypothesis that prolonged low-dose cyclophosphamide (CTX) treatment after pulse therapy attenuate paraquat (PQ)-induced lung injury in rats. METHODS: PQ (25 mg/kg) was administered intraperitoneally to induce PQ-intoxicated rat model. The rats were randomly divided into four groups: control group (1 mL/day saline solution for 14 days), PQ group (1 mL/day saline solution for 14 days after PQ exposure), pulse group (15 mg/kg/day CTX in 1 mL of saline solution for 2 days and subsequent 1 mL/day saline solution for 12 days), and prolonged low-dose group (15 mg/kg/day CTX in 1 mL of saline solution for 2 days and subsequent 1.5 mg/kg/day CTX in 1 mL of saline solution for 12 days). A 14-day follow-up was conducted to determine the survival rat, and lung hydroxyproline (HYP), wet-to-dry weight ratios (W/Dc) and histopathological changes were evaluated. RESULTS: Results showed similar survival rate (55% vs. 50%, p > 0.05) between prolonged low-dose and pulse groups. Lung W/Dc (4.94 ± 0.38 vs. 5.47 ± 0.28, p < 0.01), HYP (3.34 ± 0.29 µg/mg vs. 3.65 ± 0.19 µg/mg, p < 0.001), and fibrosis score (2.69 ± 0.84 vs. 3.13 ± 0.63, p < 0.05) were lower in prolonged low-dose group than those in the pulse group. CONCLUSIONS: These findings suggested prolonged low-dose CTX treatment after pulse therapy could attenuate PQ-induced lung injury in rats.
Subject(s)
Animals , Rats , Cyclophosphamide , Fibrosis , Follow-Up Studies , Hydroxyproline , Lung Injury , Lung , Models, Animal , Paraquat , Sodium Chloride , Survival RateABSTRACT
Objective To investigate the effect of a stabilization device for maintaining the balance of a cardiopulmonary resuscitation (CPR) performer during ambulance transportation on quality of CPR in out-of-hospital cardiac arrest (OHCA).Methods A prospective randomized controlled trial was performed. 167 OHCA patients with cardiac arrest (CA) time < 10 minutes admitted to Cangzhou Central Hospital from October 2014 to January 2017 were enrolled, and divided into armed stabilization device group (n = 86) and unarmed stabilization device group (n = 81) by random number table. Restoration of spontaneous circulation (ROSC) rate, 24-hour survival rate and survival rate of discharge were evaluated.Results Compared with unarmed stabilization device group, ROSC rate (29.1％ vs. 9.9％,χ2 = 9.691,P = 0.002), 24-hour survival rate (20.9％ vs. 6.2％,χ2 = 7.649,P = 0.006) and survival rate of discharge (12.8％ vs. 3.7％,χ2 = 4.485,P = 0.035) were significant increased in armed stabilization device group. Conclusion CPR with stabilization device during ambulance transport could effectively ensure quality of CPR and improve prognosis in OHCA.Clinical Trial Registration Chinese Clinical Trial Registry, ChiCTR-IPR-14005337.
ABSTRACT
Objective To investigate the effect of a stabilization device for maintaining the balance of a cardiopulmonary resuscitation (CPR) performer during ambulance transportation on quality of CPR in out-of-hospital cardiac arrest (OHCA).Methods A prospective randomized controlled trial was performed. 167 OHCA patients with cardiac arrest (CA) time < 10 minutes admitted to Cangzhou Central Hospital from October 2014 to January 2017 were enrolled, and divided into armed stabilization device group (n = 86) and unarmed stabilization device group (n = 81) by random number table. Restoration of spontaneous circulation (ROSC) rate, 24-hour survival rate and survival rate of discharge were evaluated.Results Compared with unarmed stabilization device group, ROSC rate (29.1％ vs. 9.9％,χ2 = 9.691,P = 0.002), 24-hour survival rate (20.9％ vs. 6.2％,χ2 = 7.649,P = 0.006) and survival rate of discharge (12.8％ vs. 3.7％,χ2 = 4.485,P = 0.035) were significant increased in armed stabilization device group. Conclusion CPR with stabilization device during ambulance transport could effectively ensure quality of CPR and improve prognosis in OHCA.Clinical Trial Registration Chinese Clinical Trial Registry, ChiCTR-IPR-14005337.