Breeding of highly productive γ-linolenic acid mutant strain of Mortierella ramanniance / 中国生化药物杂志

Purpose　The aim is to breed a highly productive γ-linolenic acid mutant strain.Methods　The original strain Mortierella ramanniance AS 3.3413 was treated by 5-FU,UV,LiCl as mutanting agent.Results　A highly productive γ-linolenic acid mutant strain F5 was successfully obtained.The γ-linolenic acid yield of mutant strain F5 was increased by 300% as compared with that of the original strain.Pass-generation test showed that the heredity character of mutant strain F5 was stable.After the optimal medium and culture condition was established, the yield of γ-linolenic acid was up to 1 153.63 mg/L,and was increased by 335.87% as compared with that of the original strain.Conclusion　The mutant strain can be used for the industrial production of GLA.