Experimental Research of Therapeutic Effect of the Capsaicin on the Hypermyotension in the Paralyzed Limbs / 中国康复理论与实践

@#In order to observe the changes of myotension in the paralyzed limbs after subcutaneous injection(S.C)of capsaicin,we observed the changes of the spontaneous Electromyography(EMG)recorded in the quadriceps femoris before and after T-5 level spinal transection and found that the amplitude of EMG kept a low level in normal rats but increased progressively after operation.After treating the animals with capsaicin 200mg/kg(S.C)in three consecutive days,we found a much lower spontaneous EMG and a much higher pain threshold than that of the controls.Radioimmunnoassay detected that the content of SP in spinal dorsal horn was reduced by 38.4%,the general condition did not severely changed during the experiment.The results indicate that SP in the spinal dorsal horn participates in the regulation of myotension,and it is suggested that subcutancous injection of capsaicin may be one effective treatment to reduce the hypermyotension in the paralyzed limbs.

THE BEHAVIOR OF LYMPHATIC METASTASIS OF WALKER CARCINOSARCOMA 256 IN RATS / 解剖学报

Walker carcinosarcoma 256 cells were injected intradermally into the medialaspect of right footpad of rats.After injection,the drained lymph nodes and overthe area of route of entry were examined microscopically in different period,inorder to determine the positive rate of appearance of cancer cells and metastasis inlymph nodes as well as other pathological changes.In the local area of inoculation cancer cells were noticed passing through spacesbetween the endothelial cells by ameboid movement into capillary lymph-vessels ormoved along with the lymph stream and get in to the capillary lymph-vessels fromtheir open ends.Individual or clumped cancer cells were seen in lymph-vesselsunder the epithelium and in the afferent lymph-vessels of lymph nodes.One hourafter the injection individual cancer cells were found first in the popliteal lymph nodes,then in the lumbar or renal lymph nodes on the third day.These cancer cellsappeared in the subcapsular sinuses at first,then in the intermediate and medullarysinuses subsequently and might be passed from one lymph node to another throughthe efferent lymph-vessels,for instance,cancer cells in popliteal nodes might migrateto lumbal or renal lymph nodes.When the cancer cells in lymph nodes became amass of a certain size associating with mitotic figures,a metastatic focus was thenestablished.Within twelve days after the inoculation,the total positive rates ofcancer cells and metastasis present in the popliteal lymph nodes were 59.5% and thetotal positive rate of multiple metastasis present simultaneously in several lymphnodes was 18.9 per sent.In addition to metastasis,these lymph nodes showed also themselves some im-mune changes,as including proliferation and enlargement of lymph follicles in cortex;widening of the paracortical area;ameboid movement present in different forms ofsmall lymphocytes and reticulosis,transformation of plasmacytes as well as increaseof immunoblasts.