ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol in Liuhe Dingzhong Pills (Aurantii Fructus,Citri reticulatae Pericarpium,Magnoliae officinalis Cortex,etc.).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Dikma Spursil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 283 nm and 294 nm.RESULTS Six constituents showed good liner relationships within their own ranges (r≥0.999 3),whose average recoveries were 92.6％-103.7％ with the RSDs of 0.89％-2.87％.The contents of naringin and neohesperidin in three batches of samples showed obvious differences.CONCLUSION We should pay attention to the unstable quality of Liuhe Dingzhong Pills due to Aurantii Fructus.