Purification and activity of anti-PD-L1&CXCR4 bispecific nanobody / 中国药科大学学报

@#Targeted programmed death-ligand 1 (PD-L1) and CXC chemokine receptor type 4 (CXCR4), gene sequences encoding anti-PD-L1 nanobody and anti-CXCR4 nanobody were cloned into the pET-22b (+) vector to construct recombinant expression plasmid of anti-PD-L1&CXCR4 bispecific nanobody, which was connected with 6 × His tag and transformed into E.coli BL21 (DE3). The expressed proteins were then found to exist as a soluble form in the supernatant of bacterial lysate after induction of IPTG.Three purification methods were used to obtain the target protein in order to improve the yield and purity of the bispecific nanobody.The bacterial supernatant was separated and purified by His Trap FF affinity chromatographic column.The target protein output could exceed 1 mg/L, and the product purity could reach up to 97%.Besides, the anti-PD-L1&CXCR4 bispecific nanobody shows a specific binding ability to two antigens on the cell surface, enhancing the cytotoxicity of IL-2 activated human peripheral blood mononuclear cells (PBMC) to tumor cell line AsPC-1, which lays the foundation for further evaluation of its drug efficacy in vivo.

Hospital service optimization practice based on third-party satisfaction measurement / 中华医院管理杂志

The paper introduced the third-party satisfaction measurement and refined appraisal for quality continuous improvement programs launched by the hospital,and the PDCA management tools which optimized quality of care and patient satisfaction.The hospital is thus recommended to leverage databases for big data comparison,and carry out refined management on such basis,for the purposes of better performance management,higher doctor-patient friendship and patient trust.

Internal fixation with Kirschner wire manipulation and suture traction reduction for ulnoradial fractures / 中华创伤骨科杂志

Objective To study internal fixation with Kirschner wire manipulation and suture traction reduction for the treatment of ulnoradial fractures.Methods From January 2013 to December 2016,50 patients with ulnoradial fracture were treated at Orthopaedic Department,Yuyao Chinese Traditional Medicine Hospital.They were divided into 2 equal groups (n =25).In the control group,there were 13 men and 12 women,aged from 21 to 76 years (average,43.8 years);18 right and 7 left sides were involved;there were 14 cases of type A3 and 11 cases of type B3 according to the AO classification.In the observation group,there were 15 men and 10 women,aged from 20 to 78 years (average,44.7 years);19 right and 6 left sides were involved;there were 12 cases of type A3 and 13 cases of type B3 according to the AO classification.The control group was treated by traditional open reduction and internal fixation while the observation group by internal fixation with Kirschner wire manipulation and suture traction reduction.Fracture reduction was evaluated by the Matta criteria and functional recovery by the Berton criteria after surgery.Results The 2 groups were comparable because there were no significant differences in the general data between the 2 groups (P ＞ 0.05).Both groups were followed up for 12 to 24 months with an average of 15.5 months.Follow-ups revealed no neurological dysfunction,postoperative wound infection,improper position of internal fixation,or such complications as internal fixation loosening or breakage.All the fracture eventually healed.The excellent to good rate by the Matta criteria was 92.0％ in the control group and 96.0％ in the observation group;the excellent to good rate one year after surgery by the Berton criteria was 88.0％ in the control group and 92.0％ in the observation group.The differences were not statistically significant (P ＞ 0.05).Conclusion In the treatment of ulnoradial fractures,internal fixation with Kirschner wire manipulation and suture traction reduction is recommendable because its procedures are easy,need simple materials and can be carried out in a common operative theater.

Effect of puerarin on uterine estrogen receptor ? expression in ovariectomized rats / 中华中医药杂志

Objective:To explore the effect of puerarin on uterine estrogen receptor(ER)? expression in ovariectomized rats.Methods:Ovariectomized rats were given with puerarin in different doses and premarin for 30 days,and then the expression of E2 was observe by ELISA and the expressions of uterine ER? were observed by using immunohistochemical methods.Results:The expressions of serum E2 in ovariectomized rats were decreased(P