ABSTRACT
Type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD) are the most problematic metabolic diseases in the world. NAFLD encompasses a spectrum of severity, ranging from simple steatosis to non-alcoholic steatohepatitis (NASH) and fibrosis, increasing the risk of cirrhosis and hepatocellular carcinoma. Importantly, NAFLD is closely linked to obesity and tightly interrelated with insulin resistance and T2DM. T2DM and NAFLD (T2DM-NAFLD) are called as the Xike Rixijing Disease and Tonglaga Indigestion Disease respectively, in Mongolian medicine. Xike Rixijing Disease maybe develop into Tonglaga Indigestion Disease. Forturnately many Mongolian medicines show efficient treatment of T2DM-NAFLD, such as Agriophyllum squarrosum, Haliyasu (dried powder of camel placenta), Digeda-4 (herbs of Lomatogonium carinthiacum, rhizomata of Coptis chinensis, ripe fruits of Gardenia jasminoides, herbs of Dianthus superbus), Guangmingyan Siwei Decoction Powder (Halite, ripe fruits of Terminalia chebula, rhizomata of Zingiber officinale, fruit clusters of Piper longum), Tonglaga-5 (ripe fruits of Punica granatum, barks of Cinnamomum cassia, ripe fruits of Amomum kravanh, fruit clusters of Piper longum, flowers of Carthamus tinctorius), Tegexidegeqi (rhizomata of Inula helenium, ripe fruits of Gardenia jasminoides, rhizomata of Platycodon grandiflorum, rhizomata of Coptis chinensis, heartwood of Caesalpinia sappan), Ligan Shiliu Bawei San (ripe fruits of Punica granatum, barks of Cinnamomum cassia, ripe fruits of Amomum kravanh, fruit clusters of Piper longum, flowers of Carthamus tinctorius, ripe fruits of Amomum tsao-ko, rhizomata of Zingiber officinale), etc. Principles of Mongolian medicine in treating diseases: by balancing “three essences or roots” and “seven elements”, strengthening liver and kidney function, transporting nutrients to enhance physical strength and disease resistance, and combined with drugs for comprehensive conditioning treatment. However, their molecular mechanisms remain unclear. In this review, we prospect that Mongolian medicines might be a promising treatment for T2DM-NAFLD by activating P2X7R/NLRP3/NF-κB inflammatory pathway via lipid-sensitive nuclear receptors (i.e., FXR and LXR).
ABSTRACT
Aim To observe the effect of agiophyllum oligo saccharides ( AOS) on reducing blood sugar, im-proving insulin resistance on diadetic Goto-Kakizaki ( GK) rats, and to explore the possible mechanism. Methods The type 2 diabetes GK rats were divided into six groups: model control group ( MC ) , Glenn benzene urea group ( GLB ) , high agriophyllum squar-rosum coarse oligosaccharides ( AOS-H ) , medium ( AOS-M ) , low dose group ( AOS-L ) , homologous Wistar rats as normal control ( NC ) . All animals were administered with AOS by oral gavage, for 8 weeks. The fasting blood glucose ( FBG) , random blood sugar ( RBG) , glucose tolerance ( OGTT) were tested before and after administration. No fasting sugar load status before and after dosing changes in blood glucose and serum insulin level in rats were measured in the previ-ous 8 weeks. At the end of administration, the fasting serum glucose ( FPG) , insulin ( FINS) , OGTT and in-sulin resistance index ( HOMA IR) in fasting rats were analyzed. Lastly, the pathological changing of pancreas was observed by HE staining. Results The blood glucose of fasting GK rats was not influenced after using AOS. However, the random blood glucose significantly reduced, the glucose tolerance was improved and AUC was obviously reduced (P < 0. 01) after using AOS. The best effect was on AOS-M group, which was similar with Glenn benzene urea. Through our research, we found AOS could promote release of insulin. This best effect was on AOS-M and AOS-L groups, and the time and quantity of release were better than Glenn benzene urea. Finally, AOS inhibited the pathological changes of islet tissue on GK rats, increased the quantity of pancreas and islet cells. Compared with model group, the changing of islet structure was significantly reduced in AOS group. Conclusion AOS could obviously improve insulin resistance and lower blood sugar, and the mechanism of this effect may be related with rapidly promoting insulin release, increasing the islet cell proliferation,and improving the function of islet.