ABSTRACT
Objective:To establish an HPLC method for the determination of the content of magnolol and honokiol in Cortex mag-noliae Officinalis formula granules and compare the content of the formula granules from different manufacturers. Methods:An HPLC was used to determine the content of magnolol and honokiol in Cortex magnoliae Officinalis formula granules. The analysis was carried out on a Hypersil C18 (250 mm × 4. 6 mm, 5 μm) chromatographic column. Acetonitrile-water was used as the mobile phase with gra-dient elution and the flow rate was 1. 0 ml·min-1 . The detection wavelength was set at 294 nm, the sample size was 20 μl and the column temperature was 25℃. Results:The linear range was 0. 873-26. 190μg·ml-1(r=0. 999 5) for magnolol, the average recov-ery was 99. 24% with RSD of 2. 00%(n=6) and that was 0. 732-21. 980μg·ml-1(r=0. 999 0) for honokiol,and the average recov-ery was 99. 89% with RSD of 1. 33%(n=6). The difference in the content of magnolol and honokiol in Cortex magnoliae Officinalis formula granules from different manufacturers was notable. Conclusion: The method is simple, repeatable and feasible, and can be used for the quality control of magnolol and honokiol in Cortex magnoliae Officinalis formula granules. The content difference in magno-lol and honokiol in Cortex magnoliae Officinalis formula granules from different manufacturers suggests that it is necessary to standardize the planting and selecting of Chinese medicine, and develop scientific and unified production technology and quality standard for the formula granules.
ABSTRACT
Objective To investigate the clinical value of high frequency color Doppler ultransound in qualitative diagnosis of breast cancer.Methods 39 patients of breast cancer received the examinnation of high frequency color ultrasound were retrospectively analyzed,obeserving the location,the fore,the border,the longitud-latiude ratio,the echo intra and behind minor lesions,the ting calcification points and the axillary lymphodes.Color Doppler ultrasound emphasizes the blood steam characters of breast tumor.Results 39 patients with breast cancer acoustic image secks the specific feature,the form was mang,the border isn't clear,the internal echo was not even occupy 72％.It could be seen part ring kichen store calcifies.Signal of bloodstream it was mang to enrich over Ⅱ grade,RI ＞ 0.7 ultrasonic diagnosis in its owns group 35 evamples.Two patiens the property of example remained to be checked.One patien diagnosis of example was tubercle of prohferation,and one patien diagnosis of example was fibroma of manmary gland.The total diagnostic rate of coincidence by ultrasonograms was 89.7％,and the rate of missed diagnosis was 5.2％.Conclusion High frequency color dopple ultransound had great diagonstic values in brease cancer.