ABSTRACT
Objective@#To explore the key technique and outcome of transoral radiofrequency ablation microsurgery for early stage of glottic carcinoma with anterior commissure involvement (ACI).@*Methods@#A retrospective analysis was conducted on 31 patients, who were diagnosed as early stage glottic carcinoma during January 2010 to March 2016 in ENT Department. According to whether the anterior commissure was involved or not, two groups were divided. There were eleven cases with ACI (stages T1a, T1b, and T2). Twenty cases without ACI (stages Tis, T1a, and T2). All the patients received transoral radiofrequency ablation microsurgery and followed up closely.Only one case received radiotheraphy after surgery. SPSS19.0 software was used to analyze data.@*Results@#The follow-up time was 12-67 months, and the median follow-up time was 30 months. Nine among 11 cases with ACI obtained good oncologic outcomes, initial local recurrence was identified in 2/11 cases, including 2 cases of T2. Two cases ultimately required salvage total laryngectomy. Meanwhile, initial local recurrence was identified in 2/20 cases without ACI, including 1 case of T1a and 1 case of T2. One case underwent elective neck dissection, and another one received salvage total laryngectomy.Compared to the patients without ACI, it seemed that the cases with ACI always accomponied with a little higher initial local recurrence and lower overall laryngealpreservation, but the difference had no significance (P>0.05).@*Conclusions@#Transoral radiofrequency ablation microsurgery is an effective treatment for glottic carcinoma with ACI. Its advantages, such as more flexibility and deformability, make it more feasible to operate at the narrow space of anterior commissure assisted with laryngeal endoscopy.Good oncologic outcomes can be obtained by this technique with lower initial local recurrence as well as higher overall laryngeal preservation rate.
ABSTRACT
OBJECTIVE@#To investigate the expression of TGF-beta1 and HSP70 in human laryngeal squamous cell carcinoma.@*METHOD@#The expression levels of TGF-beta1 and HSP70 in 53 specimens of human laryngeal squamous cell carcinoma and 48 specimens of para-carcinoma were detected by immuno- histochemistry and computer assisted image analysis.@*RESULT@#The expression of TGF-beta1 was decreased in carcinoma tissues compared with para-carcinoma tissues ( P < 0.05), while the expression of HSP70 was increased ( P < 0.05). Both the expression of TGF-beta1 and HSP70 were significantly correlated with the differentiation of laryngeal squamous cell carcinoma (P < 0.01 or P < 0.05). However, there was a negative correlation between TGF-beta1 and HSP70 (r = -0.87, P < 0.01).@*CONCLUSION@#TGF-beta1 and HSP70 play an important role in malignant behaviors of human laryngeal carcinoma.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , HSP70 Heat-Shock Proteins , Metabolism , Immunohistochemistry , Laryngeal Neoplasms , Metabolism , Pathology , Neoplasm Staging , Prognosis , Transforming Growth Factor beta1 , MetabolismABSTRACT
Objective To localize the gene of a Chinese autosomal-dominant non-syndrome deafness (DFNA) family by linkage analysis. Methods Pedigree was drawn after genetic investigation. All family members were checked up, and detail audiology examination proceeded (including pure tone audiometer, acoustic immittance measurement, auditory brainstem response). Vestibular function was evaluated. At first, the similarity of the family's locus linkage with the known DFNA loci should be excluded, and then whole genome scan was carried out. Results Deafness gene of this family was localized at 2q13-q14.2. A maximum LOD score of 3.22 (?=0.00) was obtained with the microsatellite marker D2S363. Haplotype analysis placed the novel locus within a 8.4cM region interval defined by markers D2S1888 and 2S2224. DNA sequencing of coding regions and exon/intron boundaries of a candidate gene PAX8 in this interval did not reveal disease-causing mutation in this family. Conclusion A novel DFNA locus was defined in a Chinese Neimeng family.