ABSTRACT
Aim: The common non-steroidal anti-inflammatory drugs (NSAIDs) and opioids have adverse effects. This has motivated for the search of new drugs with decreased or no side effects. In the present study, we investigated analgesic and anti-inflammatory activities of the methanol extract of Tithonia diversifolia in vivo. Methods: Analgesic activity of the methanol extract of T. diversifolia was carried out using an analgesy meter to measure the tolerance of induced pressure. The method of carrageenan-induced inflammation in rat was used for the anti-inflammatory effect. Results: This study shows statistically significant improvements in pain resistance and paw oedema suppression were observed in animals treated with 150 and 300 mg/kg body weight (b.w.) methanol extract of Tithonia diversifolia, when compared to control groups treated with normal saline and acetyl salicylic acid. The maximum analgesic effect was achieved at 300 mg/kg after 30 min with effective ratio of 5.92 when compared with the standard drug acetyl salicylic acid, and twice the activity of 150 mg/kg at the same time. Conclusion: The results suggest that the plant extract has analgesic and anti-inflammatory activities, supporting its uses in traditional medicine.
ABSTRACT
Background: This study investigated the hypoglycemic potential of methanolic extract of Passiflora foetida (PF) in alloxan-induced diabetic albino mice. Diabetes is a metabolic disease characterized with high blood sugar levels over a prolonged period. Discovery of a new drug will greatly reduce the mortality and morbidity associated with the disease. Methods: Diabetes was induced in albino mice by administration of 150 mg/kg body weight (b.w.) alloxan. Different concentrations of the methanolic extract of PF was prepared and administered orally to groups of alloxan-induced diabetic mice. Blood glucose was determine at different time point over 4 hrs. Results: The extract reduced blood glucose levels in diabetic mice significantly (P<0.001) and the kinetic parameters such as, area under glucose concentration time curve (AUC0-4hG) (P<0.05), glucose mean residence time (MRTG), glucose t1/2G were significantly lower (<0.05) in PF treated groups when compared with the control groups. The rate of glucose clearance (CLG) was high in the group treated with the extract. Conclusion: The results of this study indicate presence of hypoglycemic constituent in the plant.
ABSTRACT
Objective: The aim of the present study was to investigate the antidiabetic potential of Pergularia daemia methanolic extract (PD) on alloxan-induced diabetic mice. Methods: Diabetes was induced using alloxan (150mg/kg). Different doses of the methanolic extract of P. daemia were prepared and administered orally. The alloxaninduced diabetic mice were treated with 200, 400 and 1000 mg/kg body weight (b.w.) methanolic extract of Pergularia daemia and the blood glucose levels were measured over 4 hrs. Results: All doses of the extract (200, 400 and 1000 mg/kg b.w) significantly (p<0.0001) lowered fasting blood glucose level after treatment. The hypoglycaemic effect of the methanolic extract was also compared with that of an oral dose of glibenclamide under the same conditions. The extract showed significant (p<0.05) hypoglycemic effect in alloxan-induced diabetic mice. The glucose half-life (t1/2G) (p<0.0001), Mean Glucose Residence Time (MRTG) (p<0.0001), and the area under glucose concentration time curve (AUC0-4hG) (p<0.05) were high in control group compared to the alloxan-induced mice treated with P. daemia extract. Conclusion: The results of this study indicate that methanolic extract of the plant of Pegularia daemia possess hypoglycaemic activity.