ABSTRACT
Objective: To establish a method for the simultaneous determination of geniposide, liquiritin, aloe-emodin, rhein, emodin, chrysophanol and physcion in Yinzhi Quhuang capsules by an HPLC wavelength switching method. Methods:A Waters Sun-fire C18(250 mm ×4.6 mm,5μm) column was used with the mobile phase of methanol-0.05% phosphoric acid solution (gradient elu-tion) at a flow rate of 1. 0 ml·min-1 , the column temperature was 25℃, the detection wavelength was 238 nm in 0-15 min for genipo-side and liquiritin, and 254 nm in 15-50 min for aloe-emodin,rhein, emodin, chrysophanol and physcion. The injection volume was 10μl. Results: The linear range was 0.13-3.18 μg·ml-1 (r =0.9998) for geniposide, 0.19-4.84 μg·ml-1(r =0.9997) for liquiritin, 0. 28-7. 02μg·ml-1(r=0. 9999) for aloe-emodin, 0. 13-3. 16μg·ml-1(r=0. 9999) for rhein, 0. 61-15. 27μg·ml-1 (r=0.9999) for emodin, 0.32-8.03 μg·ml-1(r=0.9999) for chrysophanol and 0.39-9.81 μg·ml-1(r=0.9999) for phy-scion. The average recovery was 98. 84%(RSD=0. 74%), 99. 34%(RSD=0. 86%), 99. 54%(RSD=0. 30%), 99. 56% (RSD=0. 80%), 99. 85% (RSD=0. 41%), 99. 57% (RSD=0. 70%) and 99. 64% (RSD=0. 30%) (n=9). Conclusion: The deter-mination method has the advantages of simple operation, high specificity, good reproducibility and accurate results, which can be used for the quality control of Yinzhi Quhuang capsules.