ABSTRACT
Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic oxacillin-resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.
Subject(s)
Animals , Penicillin-Binding Proteins/genetics , Staphylococcus/genetics , Anti-Bacterial Agents/pharmacology , beta-Lactam Resistance , Ear/microbiology , Genes, Bacterial , Horses , Nasal Mucosa/microbiology , Oxacillin/pharmacology , Rodentia , Skin/microbiology , Staphylococcus/isolation & purificationABSTRACT
The occurrence of the sugarcane weevil Sphenophorus levis Vaurie is important in sugarcane in some regions in Brazil. Damage is caused by the larvae as they bore into the nodes and can reach 30 ton/ha/year. Many control alternatives have been attempted, but none were satisfactory, except for the use of toxic baits. Therefore, it is necessary to optimize their efficiency or to propose new techniques. The objective of the current study was to evaluate the attractiveness of adults of S. levis to sugarcane nodes and pineapple peelings in an "Y" tube olfactometer. The sugarcane internodes were treated with 10 percent molasses, and tested after different periods of fermentation (24, 48 e 72h), at different times of the day (diurnal and nocturnal) and with both sexes. These tests were carried out in order to correlate the response of S. levis to ethyl acetate and ethanol release as a result of the fermentation process. The release of both compounds was monitored by gas chromatography-mass spectrometry (GC-MS). Our data indicated that sugarcane internodes mixed with 10 percent molasses fermented for 24h and 48h were the most attractive to S. levis (up to 90 percent). Pineapple peelings attracted 62.5 percent of the tested insects. The olfactory response was higher during the day, and no differences were found between the sexes. The production of ethanol in all plant substrates was higher than ethyl acetate, but we could not establish a clear correlation with the insect response to baits.
A ocorrência do besouro da cana-de-açúcar, Sphenophorus levis Vaurie, é importante em algumas regiões do Brasil. O dano é causado pelas larvas que perfuram os colmos, ocasionando perdas de até 30 ton /ha/ano. Várias alternativas de controle têm sido utilizadas, embora nenhuma delas com resultados satisfatórios, à exceção do uso de iscas tóxicas. Sendo assim, há necessidade de otimizar sua eficiência ou propor novas técnicas. O objetivo do presente estudo foi avaliar a atratividade dos adultos de S. levis a toletes de cana e casca de abacaxi por meio de olfatômetro em "Y". Os entrenós foram tratados com melaço a 10 por cento e foram testados depois de diferentes tempos de fermentação (24, 48 e 72h), em diferentes horários (diurno e noturno) para ambos os sexos. Os testes foram conduzidos na tentativa de relacionar a resposta de S. levis ao acetato de etila e etanol produzidos pela fermentação. A produção de ambos compostos foi determinada por cromatografia gasosa de massa (GC-MS). Os dados obtidos indicaram que os entrenós da cana-de-açúcar misturados com melaço a 10 por cento e fermentados por 24h e 48h foram os mais atrativos a S. levis (acima de 90 por cento). A casca de abacaxi só atraiu 62,5 por cento dos insetos testados. A resposta olfativa foi maior durante o dia e não houve diferenças entre os sexos. A produção de etanol em todos os substratos foi maior do que a do acetato de etila, embora não tenha sido possível estabelecer uma relação clara com a resposta dos insetos às iscas.