ABSTRACT
ABSTRACT Objective The establishment of reference values for a subset of leukocytes is common in clinical practice, and ethnic variations are strongly associated with disease development. In Brazil, indigenous people are vulnerable to infections, and few studies have described the health and disease conditions of this population. This study aimed to provide reference values for immunological cell subsets in indigenous Brazilians living in the state of Mato Grosso do Sul. Methods Flow cytometry and 4-color combinations of monoclonal antibodies were used to characterize cells. A total of 115 healthy adults, mostly females (72%), were included in the study. The results are presented as mean and median (2.5%-97.5% percentiles) for T and B lymphocytes, CD4+ T cells, CD8+ T cells, Natural Killer cells, monocytes, and dendritic cells, providing an average immunological profile for the population in question. Results The relative medians of CD3+, CD4+, and CD8+ T cells were significantly higher in women than in men in a healthy indigenous population. Conclusion To our knowledge, cell reference data from indigenous Brazilians are unknown in the literature. The immune cell results presented in this pioneering study will contribute to the clinical and laboratory evaluation of the Brazilian indigenous population, especially given the important differences when compared with other Brazilian ethnic groups.
ABSTRACT
A limitação nas atividades de vida diária (AVD) por dispneia é um achado comum nos pacientes com insuficiência cardíaca (IC), classe funcional III e IV. A avaliação específica da limitação nas AVD poderia ser utilizada como parâmetro de evolução da doença e de resposta terapêutica. Entretanto, há uma escassez de instrumentos de avaliação das AVD nessa população. Dessa forma, o objetivo do estudo foi verificar a aplicabilidade da escala London Chest Activity of Daily Living (LCADL) e do teste de AVD-Glittre (T Glittre), na avaliação da limitação funcional de indivíduos com IC classe funcional III e IV. Participaram do estudo 10 pacientes (57 ± 9 anos; 27,5 ± 4,5kg/m²) de ambos os sexos com diagnóstico clínico de IC classe funcional III e IV e fração de ejeção do ventrículo esquerdo (FEVE) 34 ± 7 por cento, foram avaliados: espirometria, índice de massa corpórea (IMC), escala LCADL, teste de caminhada de seis minutos (TC6min), T Glittre, escala Medical Research Council (MRC) e questionário SF-36. Os pacientes apresentaram, em média, escore da escala LCADLtotal de 27,7 ± 12,1 (LCADL por centototal: 41,5 ± 16,9) e tempo do T Glittre de 6,3 ± 4,8 minutos, encontrando-se correlação positiva entre eles (r = 0,88; p < 0,05). O LCADL por centototal correlacionou-se com o TC6min (r = -0,83), FEVE (r = -0,64), MRC (r = 0,68) e domínio capacidade funcional (CF) do SF-36 (r = -0,63), com p < 0,05. O T Glittre correlacionou-se com o TC6min (r = -0,90), FEVE (r = -0,66) e CF do SF-36 (r = -0,69), com p < 0,05. Conclui-se com o estudo que a escala LCADL e o T Glittre têm aplicabilidade em pacientes com IC classe III e IV, apresentando associação com a FEVE, com a distância percorrida no TC6min, grau de dispneia e qualidade de vida.
Limitation in activities of daily living (ADL) caused by dyspnea is a common finding in patients with heart failure (HF), functional class III and IV. Specific assessment of ADL limitation could be used as a parameter of the disease progression and the therapy response. However, there is a shortage of instruments to assess ADL in this population. This study aimed to determine the applicability of the London Chest Activity of Daily Living (LCADL) and the Glittre ADL-test (T Glittre), to evaluate functional limitations of individuals with HF functional class III and IV. Ten patients (57±9 years, 27.5±4.5kg/m²) of both genders with a clinical diagnosis of HF functional class III and IV and left ventricle ejection fraction (LVEF) 34±7 percent participated in the study. Spirometry, body mass index (BMI), LCADL, six-minute walking test (6MWT), T Glittre, Medical Research Council Scale (MRC) and SF-36 were performed. The patients had an average score of the LCADLtotal from 27.7±12.1 (LCADL percenttotal: 41.5±16.9) and time of T Glittre 6.3±4.8 minutes. A positive correlation was found between them (r = 0.88, p<0.05). LCADL percenttotal correlated with 6MWT (r =-0.83), LVEF (r =-0.64), MRC (r =0.68) and Functional Capacity (FC) of the SF-36 (r =-0.63) (p<0.05). T Glittre correlated with 6MWT (r =-0.90), LVEF (r =-0.66) and CF of the SF-36 (r =-0.69) (p<0.05). In conclusion, the LCADL scale and T Glittre have applicability in patients with HF class III and IV, demonstrating association with LVEF, distance on the 6MWT, degree of dyspnea and quality of life.