ABSTRACT
The aim of the present study was to evaluate the antibacterial behavior of polypyrrole nanoparticles (PPy-NPs) in water against biofilm producer or not S. aureus isolated from cows and goats with mastitis. One hundred and thirty-eight isolates of S. aureus were initially evaluated for bioï¬lm formation by spectrophotometry in microplates. In addition, the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of PPy-NPs in water for planktonic S. aureus were determined. From the bovine samples analyzed, 5 (4.46%) S. aureus isolates showed a strong biofilm production, 17 (15.18%) moderate production, 36 (32.14%) with weak production and 54 (48.21%) did not produce biofilms. Strains from goats (26) showed no bioï¬lm production in 18 (69.23%) strains and weak bioï¬lm production in 8 (30.76%) strains. The MIC and MBC of S. aureus to PPy-NPs were found in the same concentration (125µÉ¡/mL) in all strains tested, regardless of biofilm production or not. This ï¬nding provides a new insight into the interaction between PPy-NPs and S. aureus, and will offer potential beneï¬ts for the control of mastitis.(AU)
Subject(s)
Animals , Female , Cattle , Pyrroles/administration & dosage , Staphylococcus aureus/drug effects , Goats/microbiology , Mastitis/veterinary , Biofilms , Anti-Bacterial Agents/therapeutic useABSTRACT
A preliminar survey to detect antibodies against BVD/MD (NADL) and BHV1 (Los Angeles) was perfomed in five dairy goat flocks from four counties of Pernambuco State, Brazil. Two hundred and six serum samples of purebred (Saanen, Toggenburg and Alpine) and crossbred goats were tested by microplate serumneutralization test. Positive results (titre >/ 4) were recorded in 11.6 per cent and 6.8 per cent of samples test to BVD/MD and BHV 1, respectively