ABSTRACT
Objetivo: O objetivo dessa revisão sistematizada da literatura foi analisar a associação entre o uso de próteses dentárias removíveis e doenças respiratórias prevalentes. Materiais e métodos: Este estudo foi conduzido seguindo os critérios do PRISMA check-list (Preferred Reporting Items for Systematic Review and Meta-Analysis). A base de dados eletrônica selecionada foi a PubMed/MEDLINE, sem restrições do ano de publicação. Estudos prospectivos e retrospectivos (ensaios clínicos randomizados, ensaios clínicos controlados, estudos de coorte, estudos caso-controle e estudos transversais), estudos in vitro e publicados em inglês foram escolhidos como critérios de elegibilidade. Resultados: A busca inicial na base de dados obteve 553 artigos e 8 deles foram selecionados baseados nos critérios de elegibilidade e subdivididos em dois tópicos: doença pulmonar obstrutiva crônica e pneumonia por aspiração. Conclusão: Com base nos estudos avaliados existe associação entre as próteses dentárias removíveis contaminadas e doenças respiratórias... (AU)
Objective: The purpose of this review was to analyze the association between the use of removable dental prostheses and the prevalence of respiratory diseases. Methodology: This study was conducted following the criteria of PRISMA check-list (Preferred Reporting Items for Systematic Review and Meta-Analysis). The database selected was PubMed/MEDLINE, with no restrictions on the year of publication. Prospective and retrospective studies (randomized clinical trials, controlled clinical trials, cohort studies, case-control studies and cross-sectional studies), in vitro studies and published in English were selected in tehe eligibility criteria. Results: The search in the database obtained 553 articles and 8 of them were selected based on the eligibility criteria and subdivided into two topics: chronic obstructive pulmonary disease and aspiration pneumonia. Conclusion: Based on the studies evaluated, there is an association between contaminated removable dental prostheses and respiratory diseases... (AU)
Subject(s)
Pneumonia, Aspiration , Prostheses and Implants , Respiratory Tract Diseases , Pulmonary Disease, Chronic ObstructiveABSTRACT
Abstract INTRODUCTION: The aac(6')-Ib-cr and bla KPC genes are spreading among Enterobacteriaceae species, including Providencia stuartii, in some countries of world. METHODS: These genes were investigated in 28 P. stuartii isolates from a public hospital in Recife, Pernambuco, Brazil, by PCR and sequencing. RESULTS: The aac(6')-Ib-cr gene was detected in 16 resistant isolates, and the bla KPC gene was seen in 14. CONCLUSIONS: The presence of these genes in P. stuartii multi- and extensively drug-resistant isolates indicates that the resistance arsenal of this species is increasing, thus limiting the therapeutic options.
Subject(s)
Humans , Enterobacteriaceae Infections , Plasmids , beta-Lactamases/genetics , Brazil , Microbial Sensitivity Tests , Providencia , Drug Resistance, Multiple, Bacterial , Anti-Bacterial Agents/pharmacologyABSTRACT
Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection University Recife Mycologia (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
ABSTRACT
Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection 'University Recife Mycologia' (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
Subject(s)
Paclitaxel/biosynthesis , Endophytes/metabolism , Fungi/metabolism , Microbiology/organization & administration , Preservation, Biological , Endophytes/genetics , Fungi/geneticsABSTRACT
A diversidade genética de catorze cultivares de cana-de-açúcar (Saccharum oficinarum) foi acessada por meio de marcadores moleculares ISSR. Objetivou-se caracterizar molecularmente as cultivares estudadas. Foram utilizados trinta e sete primers de ISSR, dos quais, oito foram eficientes na amplificação do DNA das amostras analisadas, sendo sete primers suficientes para distinguir todas as cultivares de cana-de-açúcar envolvidas nas análises. A faixa de amplicons variou de 300 a 2000 pb. As cultivares RB 92579 e RB 863129 apresentaram maiores coeficientes de similaridade (77 por cento) enquanto as cultivares RB 961 e RB 931611 formaram o grupo com menor similaridade (22 por cento). Os resultados indicam que os marcadores ISSR foram úteis na análise da diversidade genética e geração de padrões genéticos (fingerprint), em germoplasma de cana-de-açúcar. Marcadores ISSR cultivar-específico foram obtidos com o primer UBC 817 para as 14 cultivares testadas. Num próximo trabalho, mais primers ISSR serão utilizados para buscar mais polimorfismos dessas e de outras cultivares de cana-de-açúcar.
Genetic diversity of fourteen sugarcane cultivars was accessed by ISSR molecular markers. With the aim to characterizing and validating the efficiency of these markers in the fingerprint of studied cultivars, thirty seven ISSR primers were used, from which, eight were efficient for the DNA amplification. Seven primers were efficient to discriminate the fourteen studied sugarcane cultivars. The amplicons varied from 300 to 2000 bp. The cultivars RB 92579 and RB 863129 presented higher similarity coefficient (77 percent) while the cultivars RB 961 and RB 93611 formed the group with lower similarity (22 percent). The results suggested that ISSR markers were useful in the analysis of the genetic diversity and in the fingerprint in sugarcane germosplasm. In the next step more ISSR primers will be used in order to obtain more polymorphism from these varieties and to analyze more sugarcane cultivars.