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1.
Mem. Inst. Oswaldo Cruz ; 104(4): 655-658, July 2009. graf
Article in English | LILACS | ID: lil-523736

ABSTRACT

Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8 percent), HAstV-2 (23.2 percent), HAstV-3 (3.6 percent), HAstV-4 (14.3 percent) and HAstVs -5, -6, -7 and -8 (1.8 percent each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.


Subject(s)
Child, Preschool , Humans , Astroviridae Infections/virology , Diarrhea/virology , Feces/virology , Mamastrovirus/genetics , Astroviridae Infections/diagnosis , Genotype , Mamastrovirus/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction
2.
Rev. Soc. Bras. Med. Trop ; 38(2): 153-156, mar.-abr. 2005. tab
Article in English | LILACS | ID: lil-396331

ABSTRACT

Neste estudo, foram analisadas 648 amostras de soro, provenientes de profissionais de laboratório de Goiânia-Goiás, visando a detecção de três marcadores sorológicos do VHB: HBsAg, anti-HBsAg e anti-HBcAg. As amostras HBsAg e anti-HBcAg positivas foram também analisadas para os marcadores HBeAg, anti-HBeAg e anti-HBcAgIgM. Foi observado um percentual de soropositividade para infecção pelo VHB de 24,1% sendo que 0,7% destes foram positivos para o HBsAg. O DNA viral foi detectado por PCR nas duas amostras HBsAg positivas. Dos 648 profissionais, 74,5% relataram vacinação para o VHB e, destes, 89,9% apresentaram soroconversão. Trabalho direto com fluidos biológicos bem como trabalho em serviços gerais representaram risco significativo para aquisição da infecção pelo VHB. Os resultados do estudo mostram que embora tenha havido um aumento no índice de vacinação entre os trabalhadores de laboratório, o índice de infecção pelo VHB manteve-se inalterado em relação ao tempo na região.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Hepatitis B Antibodies/blood , Hepatitis B Surface Antigens/blood , Hepatitis B virus/immunology , Hepatitis B/epidemiology , Laboratory Personnel , Biomarkers/blood , Brazil/epidemiology , DNA, Viral/analysis , Enzyme-Linked Immunosorbent Assay , Hepatitis B Vaccines , Hepatitis B/diagnosis , Hepatitis B/transmission , Polymerase Chain Reaction , Prevalence , Risk Factors , Seroepidemiologic Studies
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