ABSTRACT
1. Surgical time, amylase activiry and pancreatic protein, DNA and RNA content were measured in 38 control rats and in 104 rats injected with sodium taurocholate to induced acute pancreatitis after 21 days on one of four diets differing in protein, lipid and carbohidrate cocnent. 2. All of the parameters measured were lower in rats with pancreatitis than in controls maintained on thes same diets. Among the rats with pancreatitis, those reciving a protein-free survived longer and had significantly higher DNA, lower amylase activity and lower RNA and protein levels than those reciving a balanced diet or one that was high in protein or lipid content. 3. We conclude that acute pancratitis in rats has a more benign course in protein-undernourished animals
Subject(s)
Rats , Animals , Male , Diet , Pancreatitis/mortality , Taurocholic Acid/pharmacology , Acute Disease , Amylases/blood , Dietary Proteins , Pancreas/enzymology , Pancreatitis/chemically induced , Rats, Inbred StrainsABSTRACT
The production of monnoclonal antibodies against the HBsAg is reported. Balb/c mice immunized against a commercial vaccine were used. Upon fusion of spleen cells from an animal having a high titer with the SP2/0 myeloma cell line, we obtained 6 stable cell lines, all of the IgG1 subclass. They showed a wide range of specificities against the classical HBsAg subtypes. These monoclonal antibodies can be used as the basis for the development of new methods for the screening and study of the hepatitis B virus