ABSTRACT
Human papillomavirus (HPV) is the major causative agent of cervical intraepithelial neoplasia (CIN) and cancer. The aim of this study was to characterize HPV in women with premalignant lesions and cervical cancer using a PCR/RFLP method. We investigated 34 HPV positive women and with CIN of varying degrees in the period from January 2008 to December 2009. HPV 6b was the most common genotype detected in grade I lesions (CIN I), although other high-risk types such as HPV 53, 31 and 16 were also identified in CIN I. Statistically significant positive relationship was found between the histopathological grade of lesions and HPV risk type. The results reinforce the clinical relevance of HPV genotyping, allowing more accurate monitoring and treatment of patients with risk of developing cervical cancer or injury.
Subject(s)
Humans , Papillomavirus Infections , Uterine Neoplasms , Polymerase Chain ReactionABSTRACT
The purpose of this study was to characterize the exposure of bovine aborted fetuses from beef and dairy herds of the humid pampas of Argentina to different infectious agents by the evaluation of fetal fluid antibodies. Presence of fetal antibodies to bovine viral diarrhea virus genotype 1 (BVDV-1), bovine herpes virus type 1 (BHV-1), Leptospira interrogans, Brucella abortus, and Neospora caninum was determined. Of the 95 fetuses processed, 66 came from 49 beef herds and 29 from 12 dairy herds. The average gestational age of the aborted fetuses was 7.1 months. Antibodies to the mentioned agents were detected in 65 of the 95 fetal fluids (68.4
). In addition, antibodies to more than one infectious agent were detected in 32 fetuses (33.7
), suggesting fetal exposure to multiple antigens during gestation. There were antibodies to BVDV-1, BHV-1, N. caninum and Leptospira interrogans in 43 (45.2
) and 5 (5.2
) specimens, respectively. Antibodies to B. abortus were not detected in any of the fetal fluids. The results of this study provide information on the determination of antibodies in fluids from bovine aborted fetuses exposed to different infectious agents in the region.