HOXB13 is co-localized with androgen receptor to suppress androgen-stimulated prostate-specific antigen expression / 대한해부학회지

During the prostate cancer (PCa) development and its progression into hormone independency, androgen receptor (AR) signals play a central role by triggering the regulation of target genes, including prostate-specific antigen. However, the regulation of these AR-mediated target genes is not fully understood. We have previously demonstrated a unique role of HOXB13 homeodomain protein as an AR repressor. Expression of HOXB13 was highly restricted to the prostate and its suppression dramatically increased hormone-activated AR transactivation, suggesting that prostate-specific HOXB13 was a highly potent transcriptional regulator. In this report, we demonstrated the action mechanism of HOXB13 as an AR repressor. HOXB13 suppressed androgen-stimulated AR activity by interacting with AR. HOXB13 did neither bind to AR responsive elements nor disturb nuclear translocation of AR in response to androgen. In PCa specimen, we also observed mutual expression pattern of HOXB13 and AR. These results suggest that HOXB13 not only serve as a DNA-bound transcription factor but play an important role as an AR-interacting repressor to modulate hormone-activated androgen receptor signals. Further extensive studies will uncover a novel mechanism for regulating AR-signaling pathway to lead to expose new role of HOXB13 as a non-DNA-binding transcriptional repressor.

Assessment of Quality of Life in Lower Limb Amputees Using Short-Form 36

OBJECTIVE: To assess overall quality of life (QOL) in lower limb amputees and identify the factors affecting their quality of life. METHOD: This study was designed as an interview survey using the questionnaire. The subjects were 78 lower limb amputees (male 76, female 2) and the age matched 39 healthy adult males consisted control group. Interview questionnaire included level of education, religion, marital status, occupation and income. Depression was measured by the Beck depression inventory (BDI). The QOL was measured by the MOS 36-item short-form health survey (SF-36). The SF-36 scores of amputee group were compared with that of control group, and were compared according to the various factors. RESULTS: The mean overall scores of QOL in amputee and control group were 50.2+/- 21.7, 73.6+/- 12.7 respectively (p0.05). CONCLUSION: The QOL assessed by SF-36 indicated relatively low functional and well-being status in amputees. Depression and combined diseases would be a most important factors on QOL.