ABSTRACT
A significant fruit crop, the guava (Psidium guajava L.), is grown around the world in a wide range of tropical and subtropical climates. The shelf life of guavas is short, and they ripen quickly after being harvested. This limited shelf life necessitates effective management techniques to ensure a consistent market supply through post-harvest treatments to extend the storage life. In this study, we aimed to assess the impact of different treatments on the quality attributes of guava fruit cv. Lalit at 3-day intervals during storage. The experiment was carried out in the Postharvest Laboratory, Department of Horticulture, Banaras Hindu University, Varanasi. The findings from our investigation revealed that guava fruits harvested at the mature green stage and treated with Carboxy methylcellulose (CMC) and sodium alginate, maintained their desirable chemical and functional qualities for an extended period, up to 12 days of storage. Specifically, the post-harvest treatment using CMC (1.5%) proved to be exceptionally effective in reducing physiological weight loss and decay loss and retarding the increase in total carotenoids and lycopene content. Additionally, it delayed the decline in total soluble solids, ascorbic acid content, chlorophyll content, total antioxidant capacity and phenolic content throughout the storage period. Therefore, this treatment showed excellent results in extending the shelf life of guava fruits and maintaining their post-harvest quality by delaying the processes of ripening and senescence, lowering spoilage, and improving marketability and appearance.
ABSTRACT
Background: It is still difficult to diagnose tuberculosis as a source of abdominal pain. Due to the lack of non-invasive diagnostic testing, the diagnosis remains a problem. Determining the role of polymerase chain reaction (PCR) in ascitic fluid in the diagnosis of abdominal tuberculosis, we can conclude whether it is sensitive/specific or not and further its use in the diagnosis of abdominal tuberculosis. Objective: The objective of the study was to determine the role of PCR in ascitic fluid in the diagnosis of abdominal tuberculosis and also to correlate the ADA levels of ascitic fluid with the PCR report. Materials and Methods: This prospective comparative study includes 41 clinically suspected abdominal tuberculosis patients (age range 15–65 years) over a period of 2 years. A detailed history, clinical evaluation, and relevant investigations, including radiology, were done in all patients. Bacteriological or histological approaches had to support the diagnosis of abdominal tuberculosis, and PCR was also tested for Mycobacterium tuberculosis in ascitic fluid. Results: The mean age of patients was 31.71±12.32 years with male:female ratio was 1.4:1. PCR was positive in 24 (58.8%) cases. A receiver operating characteristic curve showed that a cutoff value of 35.3 IU/L (AUC 0.998, P < 0.001) for the ADA level produced the best results as a diagnostic test for abdominal tuberculosis, yielding the following parameter values: Sensitivity 100%, specificity 94.1%, positive predictive value 96%, negative predictive value 100%, and diagnostic accuracy 97.56%. ADA values were significantly elevated during abdominal tuberculosis, indicating that ADA can still be a valuable diagnostic tool. Conclusion: Our findings indicate that ascitic fluid PCR is a safe tool for diagnosing it and should be tried at least before surgical intervention.
ABSTRACT
Background & objectives: The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide. The disease shares clinicoradiological features with tuberculosis (TB), Nocardia and several fungal diseases, and its diagnosis is frequently delayed. The present study was performed to determine the frequency of NTM disease among TB suspects in a tertiary care centre in north India. Methods: In this prospective study, mycobacterial culture isolates from pulmonary and extrapulmonary specimens among TB suspects were tested with immunochromatographic assay (ICA). All ICA-negative isolates were considered as NTM suspects and further subjected to 16S-23S rRNA internal transcribed spacer gene sequencing for confirmation and species identification. Patients with active disease were treated with drug regimen as per the identified NTM species. Follow up of patients was done to determine clinical, radiological and microbiological outcomes. Results: Of the 5409 TB suspects, 42 (0.77%) were diagnosed with NTM disease. Patients with active disease consenting for treatment were treated and followed up. Thirty four patients had NTM pulmonary disease (NTM-PD) and the remaining eight had extrapulmonary NTM (EP-NTM) disease. Mycobacterium intracellulare and M. abscessus, respectively, were most frequently isolated from NTM-PD and EP-NTM patients. Fifteen NTM-PD and seven EP-NTM patients successfully completed the treatment. Ten patients died due to unrelated causes, five were lost to follow up and another four declined the treatment. Interpretation & conclusions: Our study showed that the frequency of NTM disease was low among TB suspects at a large tertiary care centre in north India and this finding was similar to other Indian studies. More studies need to be done in other parts of the country to know the geographical variation in NTM disease, if any.
ABSTRACT
In Ayurveda, Shalyatantra is one of the eminent branches based on six major methods of management among which Agnikarma is boon for local Vata and Kaphaja Vyadhi. Its effect can be assessed as Sthanik Karma (local action), Saarvadaihik Karma (Action throughout the body) and Vishista Karma (Special actions). Based on amount of Agni needed, the condition and site of disease, Dahanupakarana are used to produce therapeutic burns during Agnikarma Chikitsa. It can be classified according to Dravya used, site, disease, Akritiand Dhatu to be cauterized. Based on the Dagdha (Burn), it is again of four type viz. scorched burn, blistered burn, superficial burn and deep burn. Its indication is in all seasons except in summer and autumn. Indications and contraindications are well expounded in classics with detail information on Purva Karma, Pradhana Karma and Paschat Karma during Agnikarma as it is superior to every other procedure used in Ayurveda Surgery. In modern medicine, there is no use of therapeutical burn i.e., Samyak Dagdha Chikitsa but its use is in other form eg., Cauterization is used for coagulation and tissue destruction. Agnikarma is considered as the best therapy to pacify Kaphanubandha Vata Dosha because Agni possesses Ushna, Tikshna, Sukshma, Aashukari Gunas, which have anti Vataja and anti Kaphaja properties. It is done by red hot Shalaka. It gives quick relief to the patient and hence is termed as best therapy.
ABSTRACT
Background & objectives: The fluoroquinolones (FQs) group of antibiotics is the backbone drugs for the management of drug-resistant tuberculosis (TB). In routine clinical practice, drug susceptibility testing (DST) for FQs is not performed, and the patients are empirically treated. A limited information exists regarding FQs resistance among pulmonary TB cases. The present study was conducted to determine the FQs resistance among drug sensitive and drug-resistant pulmonary TB patients in a tertiary care centre in north India. Methods: A total of 1619 sputum/smear-positive specimens of pulmonary TB patients were subjected to DST for first-line drugs (FLDs) and second-line drugs. In addition, FQs DST was also performed using automated Mycobacterial Growth Indicator Tube-960 liquid culture technique. The immuno-chromatographic assay was performed to distinguish Mycobacterium tuberculosis complex (MTBC) from non-MTBC. Results: Mycobacterium tuberculosis (Mtb) was isolated in 1499 sputum specimens; 1099 culture specimens were sensitive to FLDs, 249 grew as multidrug-resistant (MDR) Mtb and the remaining 151 isolates revealed any drug resistance to FLDs. While FQs monoresistance among the FLD sensitive isolates was 3.1 per cent (35/1099), 27.3 per cent (68/249) among MDR Mtb isolates had additional FQs resistance. Interpretation & conclusions: FQs resistance among drug sensitive and MDR Mtb isolates was high in Delhi, India. Based on these findings, it is recommended that the DST for FQs should be routinely performed to avoid further amplification of drug resistance.