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Malaria and typhoid co-infections can be a serious public health issue in tropical countries leading to incorrect diagnosis due to overlapping clinical presentations of malaria and typhoid and hence, causing a delay in implementing the appropriate treatment regimen for these concurrent infections. This study reports a case of six-year-old female child co-infected with severe malaria (Plasmodium falciparum) and typhoid (Salmonella typhi) diagnosed by rapid malaria antigen test (RMAT) and blood culture respectively. Further, analysis of the chloroquine resistance gene Pfcrt for the falciparum demonstrated the presence of K76T mutant allele in pfcrt gene with high IC50 (150nM) for chloroquine (CQ) drug. The present case highlights the significance of timely identification and treatment of co-infections and also provides information about the circulating P. falciparum clinical strains.
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Introduction: Diabetes is an important public health problem,one of our priority non communicable diseases (NCDs)targeted for action by world leaders. Both the number of casesand the prevalence of diabetes have been steadily increasingover the past few decades. The global prevalence of diabeteshas nearly doubled in year 2014 since 1980, rising from 4.7%to 8.5% in the adult population. Study objective was to findout risk factors for Type 2 Diabetes Mellitus among 40 yearand above in rural areas of Varanasi.Material and methods: A community based cross sectionalstudy was conducted in rural areas of Varanasi covering 40year and above. WHO, STEP approach was used for datacollection in this study. RBS and FBS blood sugar measuredand diagnosis was confirmed by WHO criteria. χ2 test wasapplied to find significant association.Results: The mean ±SD age of the study subject was55.19+11.92 years. The prevalence of smoking, alcoholintake, fruits consumption, vegetables consumption andphysical activity was 18.3%, 22.7%, 48.1%, 99.8% and 28.8%respectively. The overall prevalence of diabetes was 6.1%.The prevalence of overweight and obesity was found 15.5%and 32.5% respectively, while prevalence of hypertension was29%.Conclusion: Study showed that the association betweenphysiological risk factors such as obesity and hypertensionwith diabetes was statistically significant (p<0.05), whilebehavioural risk factors mentioned above were not statisticallysignificant (p>0.05).
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Clothes form indispensible trade in modern society & jeans clothes are among the clothes with highest demand among youth. Most of the times the jeans fabrics are made in some industries and then they are transported to different small scale industries to make readymade clothes. Mostly these small scale industries consist of small labour force mostly involved in cutting & making of readymade jeans clothes. Most of these industries are unorganised sectors. This study intends to assess morbidity patterns workers in one of such industries invoved in cutting & making of readymade jeans clothes. Objective: To assess the morbidity pattern among the workers. Material & Methods: It is a crossectional study conducted in one of the small factory producing readymade jeans clothes. The total number of workers included was 256. The objective of the study was to assess the morbidity among the workers. The study tool used was a pre validated &pretested questionnaire, which included their demographic characteristics, general & systemic examination. Results: Among all the 256 workers in the factories, highest number i.e. 136 (53.13%) were in 20-30 year age group followed by 68(26.56%) in 30-40 year age group, 24(9.38%) each in 10-20 years & 40-50 years age group and only 4(1.56%) were in 50-60 years of age group. All of them were males. Conclusion: The health needs of the people working in these industries are high due to significant co morbidities, long working hours, lack of health awareness regarding substance abuse and safe sexual practice.
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Natural sources, particularly microbes yield active molecules that have wide application in food and pharmaceutical industries, degradation of hazardous bacterial biofilms, etc. Safety and acceptability of such drugs attract researchers’ attention for new drug discovery. Here, we explored biologically active microbial strains having therapeutic applications isolated from five different geographical areas of India. On screening, we found 10 strains capable of producing chitinase (Chi), seven cholesterol oxidase (COD), five glutaminase (Gln) and two heparinase (Hep) producing strains. Most of the isolated strains were found to be actinomycetes. Morphological and biochemical characterization of the strains suggest that the selected 13 isolates belong to the genus Streptomyces. Out of which, four were characterized through 16S ribosomal RNA gene analysis as Streptomyces xanthochromogenes MTCC 11937 (S1), Streptomyces violascens (N1), Streptomyces xanthopheus MTCC 11938 (H1) and Streptomyces rimosus MTCC 10792 (Ay). Results suggest that the soil isolated Streptomyces strains continue to act as a fascinating source of clinical and commercially importance enzymes. Partially purified enzymes were found to possess a broad range of pH and temperature stability indicating their capability to be used in clinical and pharmaceutical fields.
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Chitinase is one of the important enzymes as it is directly linked to Chitin that has wide applications in industrial, medical and commercial fields for its biocompatibility and biodegradability. Here, we report extracellular chitinase production by Streptomyces violascens NRRL B2700 under submerged fermentation condition. Chitinase production started after 10 h of incubation and reached to maximum level at 72 h of cultivation. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that maltose, xylose, fructose, lactose, soybean meal and ammonium nitrate served as good carbon and nitrogen sources to enhance chitinase yield by 1.6 to 6 fold. Medium supplemented with 1% colloidal chitin produced high chitinase concentration (0.1714 U/mg). The enzyme chitinase was purified from the culture broth by 75% ammonium sulphate precipitation, DEAE-cellulose ion-exchange and sephadex G-100 gel filtration. The molecular mass of the purified chitinase was 65 kDa as estimated by SDS-PAGE. The apparent Michaelis constant (Km) and the maximum rate (Vmax) of the enzyme for colloidal chitin were 1.556 mg/mL and 2.680 µM/min/mg, respectively suggested high affinity towards chitin. Possibly, it is the first report on production of chitinase from S. violascens NRRL B2700. The findings were encouraging, especially for cost effective production, and further warrants media and purification optimization studies for enhanced yield.
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Statistics is an integral part of Clinical Trials. Elements of statistics span Clinical Trial design, data monitoring, analyses and reporting. A solid understanding of statistical concepts by clinicians improves the comprehension and the resulting quality of Clinical Trials. In biomedical research it has been seen that researcher frequently use t‑test and ANOVA to compare means between the groups of interest irrespective of the nature of the data. In Clinical Trials we record the data on the patients more than two times. In such a situation using the standard ANOVA procedures is not appropriate as it does not consider dependencies between observations within subjects in the analysis. To deal with such types of study data Repeated Measure ANOVA should be used. In this article the application of One‑way Repeated Measure ANOVA has been demonstrated by using the software SPSS (Statistical Package for Social Sciences) Version 15.0 on the data collected at four time points 0 day, 15th day, 30th day, and 45th day of multicentre clinical trial conducted on Pandu Roga (~Iron Deficiency Anemia) with an Ayurvedic formulation Dhatrilauha.
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Antimicrobial activities of twenty bacterial strains isolated from ten different stressed agro-ecological niches of Eastern Uttar Pradesh, India were evaluated against bacteria, yeasts and molds. Eleven isolates showing strong antimicrobial activities were characterized. Eight antifungal compounds were purified and partially characterized by Ultra-Violet (UV) absorption spectra and grouped into polyenes and non-polyenes. Antibacterial metabolites produced by four isolates were purified and chemically characterized, of which one isolate (AB) produced a new form of olivanic acid, and other three isolates (C5, Py and M4) produced antibacterial compounds having phenoxazone nucleus.