ABSTRACT
Aim: The aim of the present study was to characterize the zinc oxide nano particle incorporated Chitosan (ZnO-NP-CS) and its antimicrobial activity. Methodology: Zinc oxide nanoparticles (ZnO-NP) were prepared by sol-gel method and Minimum Inhibitory Concentration (MIC), Minimum bactericidal Concentration (MBC) and agar well diffusion method was used for the assessment of antibacterial activity of ZnO-NP and ZnO-NP-CS as well. Results: In UV–spectroscopy, blue shift in wavelength (~365 nm) corresponding to bulk ZnO particles (~385 nm) indicates the nano size. In SEM image, ZnO-NP appeared as nano flake shape and ZnO-NP treated Methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa (PA) bacteria illustrates leakage of intracellular content, fusion and shrinkage of bacteria, respectively. The MIC of ZnO-NP for most of food pathogens were between 0.01 to 0.1mg. Lower MIC was observed for Vibrio cholerae and Listeria monocytogenes; higher MIC was observed for Bacillus cereus and Pseudomonas aeruginosa. In antibiogram assay, the zone of inhibition of ZnO-NP-CS was equal to commercial antibiotics against Multiple Drug Resistant bacteria. Interpretation: The combined effect of ZnO-NP and chitosan is better than the individual component, i.e., around 5–15 mm wider zone of inhibition than chitosan. ZnO-NP-CS can be a suitable alternative for the treatment of wound infected by multiple drug resistant bacteria
ABSTRACT
Neurofibromas are rare in the head and neck region, but most frequent tumor of neural origin. Oral hard and soft tissue is affected by the tumor. In this paper, we describe an unusual case of non-syndromic solitary neurofibroma of the floor of the mouth in a 70-year-female patient with a chief complaint of growth in the floor of the mouth for the past 3 months. An occlusal, intraoral periapical radiograph and CT imaging were done. After confirming the diagnosis, the lesion was excised under local anesthesia and the specimen was submitted for histopathological examination. On subsequent follow-up, the patient was asymptomatic. Intraoral neurofibroma although uncommon, deserve special attention because of their similarity with other inflammatory neoplastic condition, and their tendency to undergo malignant transformation.