Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add filters








Language
Year range
1.
China Pharmacy ; (12): 2082-2086, 2022.
Article in Chinese | WPRIM | ID: wpr-941446

ABSTRACT

OBJECTIVE To analyze quality maker (Q-marker) of Ka nggongyan soft capsule (KSC). METHODS The fingerprints of 20 batches of KSC were established by ultra high performance liquid chromatography (UPLC)method. Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition)were used to evaluate the similarity and confirm common peaks. The contents of norisoboldine ,leonurine hydrochloride ,forsythoside B ,acteoside,poliumoside and isoacteoside were determined by the same UPLC method. Targets and pathways related to KSC in the treatment of cervicitis were screened and analyzed by network pharmacology and molecular docking method to construct a “component-target-pathway”network,and analyze its potential Q-marker. RESULTS Twelve common peaks were identified in the fingerprints of 20 batches of KSC ,and the similarity was greater than 0.99. Six common peaks were identified ,including norisoboldine ,leonurine hydrochloride ,forsythoside B,acteoside,poliumoside and isoacteoside. The contents of the above 6 components were 1.336-1.774,0.093-0.143,4.970-5.888, 0.505-0.623,5.206-6.226 and 0.785-0.895 mg/g,respectively. By network pharmacology analysis ,14 key targets and 94 pathways were obtained ,and their binding energies to the core targets (protein kinase B 1,tumor necrosis factor )were all less than -6.4 kJ/cal. CONCLUSIONS Six components such as norisoboldine and leonurine hydrochloride are potential Q-marker of KSC.

2.
Tianjin Medical Journal ; (12): 1005-1009, 2016.
Article in Chinese | WPRIM | ID: wpr-496300

ABSTRACT

Objective To study the expressions of osteopontin (OPN),caspase-3 and mt-P53 proteins, and their relationship in gliomas. Methods Seventy gliomas specimens of patients (glioma group) were selected, and 10 samples of non-glioma brain tissue were used as control group. The SP method was used to detect the positive rates of protein expressions of OPN, caspase-3 and mt-P53 between two groups. The relationship between protein expressions of OPN, caspase-3 and mt-P53 in gliomas and grade of gliomas were detected by Western blot assay. Spearman rank correlation was compared between the positive expression of OPN, caspase-3 and rate mt-P53. Results The positive expression rates of OPN and mt-P53 were significantly higher in glioma group (64.29%and 60%) than those of control group (no positive expression), but the positive expression rate of caspase-3 was significantly lower than that of control group (47.14%vs. 90%, P0.05). The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53 (P<0.001), and the lower the positive expression rate of caspase-3 (P<0.001). With the increased level of glioma grade, OPN and mt-P53 protein levels were increased, but caspase-3 protein expression level was decreased. There was a negatively correlation between OPN and the positive expression of caspase-3, but there was a positive correlation between OPN and the expression of mt-P53 (rs=-0.720 and 0.722, P<0.05). There was a negative correlation between caspase-3 and mt-P53 expressions (rs=-0.556, P<0.05). Conclusion The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53, while the lower the positive expression rate of caspase-3. The study reveals that OPN, caspase-3 and mt-P53 expressions are associated with the occurrence and the progress of gliomas. The combined detection of them can contribute to the judgment of biological behavior of gliomas.

SELECTION OF CITATIONS
SEARCH DETAIL