ABSTRACT
Intestinal microbiota plays an important role in the development of immune system,es-pecially in the formation of immune response. Immune response to vaccination varies with region and popula-tion,which may be related to the differences in intestinal microbiota. This review focused on the correlation between intestinal microbiota and immune response to vaccination in order to find a new way to enhance vac-cine-induced immune response. It was revealed that intestinal microbiota might be involved in the immune responses to vaccines against rotavirus, typhoid and polio. Although probiotics, prebiotics and synbiotics could not significantly enhance vaccine-induced immune response,they might have a beneficial effect on vac-cine by regulating intestinal microbiota.
ABSTRACT
Due to the tumor malignancy or immunosuppressive treatment,patients with cancer in general are more susceptible to vaccine-preventable infections.The types,timing,dose of vaccination or even the immunization program for them may differ from those for the normal persons.At present,it is recommended to use inactivated vaccines for patients with cancer rather than attenuated live vaccines,Vaccinations should be avoided during immunosuppressive therapy;patients with cancer should receive double dosage of hepatitis B vaccines and two doses of inactivated influenza vaccines yearly.This paper summarizes the progress in clinical trials of vaccination for cancer patients in foreign countries,and provide reference for the development and implementation of vaccination strategy for cancer patients in China.
ABSTRACT
Due to the tumor malignancy or immunosuppressive treatment,patients with cancer in general are more susceptible to vaccine-preventable infections.The types,timing,dose of vaccination or even the immunization program for them may differ from those for the normal persons.At present,it is recommended to use inactivated vaccines for patients with cancer rather than attenuated live vaccines,Vaccinations should be avoided during immunosuppressive therapy;patients with cancer should receive double dosage of hepatitis B vaccines and two doses of inactivated influenza vaccines yearly.This paper summarizes the progress in clinical trials of vaccination for cancer patients in foreign countries,and provide reference for the development and implementation of vaccination strategy for cancer patients in China.
ABSTRACT
OBJECTIVE:To study the effects of paclitaxel combined with cisplatin on the proliferation,migration and invasion of thyroid cancer cells SW579 and its mechanism. METHODS:Cells were divided into blank control group,paclitaxel group (3μmol/L),cisplatin group(30 μmol/L),drug combination group(paclitaxel 3 μmol/L+cisplatin 30 μmol/L). 48 h after culture,the relative cell activity was measured by MTT assay. Cell cycle was detected by flow cytometry. Migration and invasion of cell was tested by Transwell assay. The expression of phosphatase and tensin homolog deleted on chromosome ten(PTEN),protein kinase B(AKT),Cyclin D1,p27,matrix metalloproteinase(MMP)-2 and MMP-9 were detected by Western blot. RESULTS:Compared with blank control group,relative cell activity of all treatment groups were decreased;paclitaxel or plus cisplatin also made cell cy-cle arrest in G1 phase,and migration and invasion ability of cell were decreased;the expression of PTEN and p27 remarkably in-creased,while the expression of Cyclin D1,MMP-2,MMP-9 and phosphorylation of AKT were obviously reduced,with statisti-cal significance (P<0.05). Compared with single drug group,the effect of drug combination group strengthened,with statistical significance in above indicators(P<0.05). CONCLUSIONS:The inhibition effect of paclitaxel combined with cisplatin on the pro-liferation,migration and invasion of thyroid cancer cells SW579 cell will be strengthened,by a mechanism of up-regulating the ex-pression of PTEN and p27,down-regulating the expression of Cyclin D1,MMP-2 and MMP-9,inhibiting phosphorylation of AKT.