ABSTRACT
Live attenuated fowl cholera; fowl pox turkeys to fowl cholera vaccine. and pigeon pox vaccines were used to vaccinate turkey in a mutual manner. Six groups of turkeys [20 birds/group] were used. The first group was vaccinated with live attenuated fowl cholera and fowl pox vaccines via the wing web and feather follicle respectively. The second group was vaccinated with fowl cholera and pigeon pox vaccine, while the third group was vaccinated with fowl pox alone. The fourth group was vaccinated with pigeon pox vaccine alone and the fifth group was vaccinated with live attenuated fowl cholera vaccine alone. The sixth group was kept as unvaccinated control. The results of indirect haemagglutination test [IHA] and ELISA revealed a significant increase in Pasteurella multocida antibody titers between group [1], group [2] and group [5]. It could be concluded that the use of the pox vaccines enhance the immune response of vaccinate turkey in a mutual manner
ABSTRACT
Infectious laryngotracheitis virus [ILTV] was detected in chicken laryngeal and tracheal samples from outbreaks in 4 governorates in Egypt [Kalubia, Sharkia and Gharbia]. The viral antigen was detected by AGPT and FAT in 7 isolates, which subjected to physicochemical, biological characterization and pathological investigations. Isolated ILTV produced characteristic pock lesions on CAM of Embryonated Chicken Eggs [ECEs] and characteristic intranuclear inclusion bodies by direct FAT and histopathological examination in infected CAM and tracheal smears. The isolate S3 was the most pathogenic strain [by pathogenicity test of the isolates on ECEs and susceptible chickens] and had one more protein band than the other isolates as compared to the standard virus [by SDS-PAGE]. Ether sensitivity and thermo-stability of the isolated viruses at 56[degree sign]C indicated that the local isolate loss its infectivity to ECEs after treatment with ether for 24 hours and 15 min. at 56 [degree sign]C as well as the standard control ILTV. By cross neutralization, the results revealed that the isolated strains appeared to be related to the standard ILTV through neutralization index between each other. Infectivity titre of the virus was increased by passaging it in SPF eggs till reaching the highest titre by the 5th passage
Subject(s)
Chickens , Antigens, Viral , Enzyme-Linked Immunosorbent Assay/methods , Fluorescent Antibody Technique/methodsABSTRACT
Binary ethyleneimine [BEI] was used to inactivate the local Egyptian strain of sheep pox virus. The inactivation process was applied using final concentrations of BEI at 0.5, 1, 2 and 3% for different incubation periods at 37 dgree C. The virus was completely inactivated after 7 hours incubation with by 2% BEI final concentration; the inactivated virus was adsorbed on aluminium hydroxide gel when incubated for 6 hours in a concentration 1:1. The antibody levels were estimated by virus neutralization test and ELISA. Specific antibodies appeared from the 1[st] week post vaccination and remained until the 4[th] week post challenge. The prepared vaccine was evaluated for safety, sterility and potency. The vaccine proved to be safe, sterile and inducing protection for the vaccinated lambs when challenged by the virulent sheep pox virus up to 6 months post vaccination