ABSTRACT
To study antihyperlipidemic properties of aqueous extract of Cassia fistula leaves in mice. Experimental study. This study was conducted at Pharmacology Laboratory of Islamic International Medical College, Rawalpindi, and National Institute of Health, Islamabad from February to April 2011. 40 male Balb/c mice were used which were randomly divided into 4 groups i.e. Group A, B, C and D, each group containing 10 mice. Duration of study was 8 weeks after acclimatization [1 week]. Group A served as control group [received standard mice diet], Group B as disease control [received 2% cholesterol diet] and Group C and D as Cassia fistula groups [received 2% cholesterol diet + aqueous extract of Cassia fistula leaves 250 and 500 mg/kg respectively]. At the end of 8 weeks, blood samples of all the mice were analyzed. In disease Group B, there was significant rise in the levels of serum total cholesterol, triglycerides and LDL-C and decline in HDL-C. In Group C and D [Cassia fistula groups], there was much less rise in cholesterol, triglycerides and LDL-C especially in Group D. Also, there was significant increase in HDL-C level in group D. The results demonstrate that administration of aqueous extract of Cassia fistula leaves, along with cholesterol diet, not only prevented the rise in serum cholesterol, triglycerides and LDL-C levels but also increased the HDL-C level, demonstrating antihyperlipidemic properties of the extract
ABSTRACT
Medicinal plants play a key role in preventing various diseases. Hyperlipidemia is a major contributor to the pathogenesis of cardiovascular diseases. The purpose of the present study was to assess the effect of aqueous extract of Carum carvi seeds in diet induced hyperlipidemia in rats. 2% cholesterol diet were given to rats for six weeks and rats showed high lipid levels were included in the study. Then all rats were divided into, normal control group [A], hyperlipidemia positive control group [B], and the remaining two groups [C and D] served as experimental groups. Group C hyperlipidemic experimental rats received aqueous dried extract of Carum carvi seeds at 60 mg/kg of body weight for eight weeks on daily basis. On the other hand group D rats received simvastatin at 1.0 mg/kg body weight for eight weeks. Blood samples were collected after eight weeks. The hyperlipidemic positive control group rats showed variable increase in serum triglycerides, LDL and total cholesterol levels. Serum HDL levels decreased in hyperlipidemic positive control groups. Carum carvi and simvastatin significantly decreased the levels of these parameters in rats. On comparison Carum carvi reduced lipid levels more, effectively than the simvastatin. Carum carvi constituents, especially flavonoids and carvone have strong anti-oxidant activity which might be involved in hypolipidemia. In conclusion, Carum carvi aqueous seeds extract decrease lipid levels in diet induced hyperlipidemic rats
Subject(s)
Male , Animals, Laboratory , Hypolipidemic Agents , Cholesterol , Seeds , Cholesterol, HDL , Plant Extracts , Cholesterol, LDL , Plants, Medicinal , Diabetic Nephropathies/prevention & control , Rats, Wistar , Hyperlipidemias/therapy , TriglyceridesABSTRACT
The aim of this study was to assess the effect of ethanolic extract of Syzygium aromaticum[clove] buds in alcohol induced hepatotoxicity in rats. Randomized controlled trial. Department of Pharmacology, Fatima Memorial College of Medicine and dentistry, Lahore from 1[st] July 2011 to 31[st] December 2011. The rats were divided into five groups, i.e. control group [A], ethanol positive group [B], experimental groups [C and D] and standard group [E]. Group C experimental rats received ethanol [3 g/kg body weight daily intraperitoneal injection] and ethanolic extract of Syzygium aromaticum at 250mg/kg of body weight daily orally for a period of forty five days. Group D experimental rats received ethanol and ethanolic extract of Syzygium aromaticum at 500 mg/kg of body weight. Group E received ethanol and silymarin [100mg/kg orally].Blood samples were taken at 45[th] day and liver in each was taken out for histopathological examination. The ethanol group rats showed variable increase in serum ALT [Alanine Transaminase], AST[Aspartate transaminase], ALP[Alkaline Phosphatase] and total bilirubin levels. In group C and group E rats the levels of these parameters become slightly decreased while in group D rats the levels decreased more towards normal. The morphological examination of experimental groups C and E rats showed slight recovery whereas the rats in experimental group D showed a significant recovery. Syzygium aromaticum constituents, especially flavonoids and polyphenols have strong anti-oxidant activity which provides hepato-protection against alcohol induced hepatotoxicity. High dose of Syzygium aromaticun ethanolic extract [500 mg/kg body weight] showed better hepatoprotection against alcohol induced hepatotoxicity than low dose Syzygium aromaticum extract and silymarin in rats
ABSTRACT
The purpose of the present study was to assess the effect of ethanolic extract of Syzygium aromaticum buds in streptozotocin [STZ] induced diabetes in rats. Randomized controlled trial. This study was conducted at the Department of Pharmacology, Islamic International Medical College, Rawalpindi from 1[st] Jan 2011 to 30 [st] June 2011. Single injection of STZ was given intraperitoneally to rats and rats showed fasting glucose level over 280 mg/dl were included in the study. After induction of diabetes all rats were divided into, normal control group [A], diabetes positive control group [B], and the two groups [C and D] served as experimental groups while group E served as standard as it received glibenclamide. Group C and D diabetic experimental rats received ethanolic extract of Syzygium aromaticum at 250 mg/kg and 500mg/kg of body weight orally for eight weeks on daily basis. On the other hand group E rats received glibenclamide at 0.5 mg/kg body weight orally for eight weeks. Blood samples were collected after eight weeks. The diabetic positive group rats showed variable increase in serum levels of glucose, triglycerides, low density dipoprotein [LDL] and total cholesterol levels. Serum high density lipoprotein [HDL] levels decreased in diabetic positive group. Syzygium aromaticum 250 mg/kg and 500 mg/kg dose and glibenclamide significantly decreased the levels of these parameters in rats. On comparison Syzygium aromaticum 500 mg/kg dose reduced glucose and lipid levels more, effectively than the 250mg/kg dose of Syzygium aromaticum and glibenclamide. Syzygium aromaticum constitutents, especially polyphenols and flavonoids have strong anti-oxidant activity which might be involved in glucose and lipid lowering effect. Syzygium aromaticum ethanolic extract decrease glucose and lipid levels in experimentally induced diabetic rats
ABSTRACT
To determine the Reno-protective effect of ethanolic extract of Cassia fistula [Amaltas] leaves on Streptozotocin induced diabetic nephropathy in rats. An animal experimental study. The study was done in Biochemistry department, Islamic International Medical College,Rawalpindi and NIH.Islamabad. Single injection of STZ was given intraperitoneally to rats and those rats that showed fasting blood glucose level over 280mg/dl were included in the study. After induction of diabetes all rats were divided into, normal control group [A], diabetes positive control group [B], and the two groups [C and D] served as experimental groups while group E served as standard as it received glibenclamide. Group C and D diabetic experimental rats received ethanolic extract of Cassia fistula leaves at 400 mg/kg and 500mg/kg of body weight orally for eight weeks on daily basis. On the other hand group E rats received glibenclamide at 0.5 mg/kg body weight orally for eight weeks. Blood samples were collected after eight weeks to find reno-protection against STZ induced diabetic nephropathy. The diabetic positive group rats showed variable increase in levels of serum glucose, serum urea, serum creatinine, total urinary protein and microalbuminuric levels. Body weight decreased and urine volume increased in diabetic groups. Cassia fistula ethanolic extract of 400mg/kg and 500mg/kg body weight dose and glibenclamide significantly decreased the levels of these parameters in rats. On comparison Cassia fistula ethanolic extract of 500mg/kg dose reduced levels of biochemical parameters more effectively than the 400mg/kg dose of Cassia fistula and glibenclamide. Cassia fistula constituents, especially polyphenols and flavonoids have strong anti-oxidant activity which might be involved in reno-protection. Cassia fistula ethanolic leaf extract showed reno-protection against STZ induced diabetic nephropathy in rats