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1.
Genet. mol. res. (Online) ; 6(3): 707-712, 2007. ilus, tab
Article in English | LILACS | ID: lil-498899

ABSTRACT

Cratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from Germplasm Banks of Embrapa Gado de Leite (Juiz de Fora, MG) and Embrapa Semi-Árido (Petrolina, PE), respectively. Root tips were treated with 3 mM 8-hydroxyquinoline and slides were made using the air-dry technique. Karyotype description for each accession took into account the following features: chromosome number; total length, relative length and arm ratio of each chromosome; haploid set length, and degree of asymmetry. Mitotic metaphases in both species showed 2n = 22 chromosomes, where this is the first report of diploid number for C. mollis. Chromosome length was also quite similar for the two species, ranging from 5.08 to 2.50 µm in C. argentea and 5.12 to 2.51 µm in C. mollis, with haploid sets of equal size, measuring 38.10 and 37.85 µm, respectively. However, they did not show the same karyotypic formula, which was 5 m + 4 sm + 2 st for C. argentea and 7 m + 2 sm + 2 st for C. mollis. This indicates the occurrence of rearrangements within chromosomes I and VI. Both karyotypes showed a tendency for asymmetry.


Subject(s)
Azure Stains , Chromosomes, Plant/metabolism , Fabaceae/genetics , Cytogenetics , Karyotyping , Metaphase
2.
Arq. bras. med. vet. zootec ; 57(6): 830-834, dez. 2005. tab
Article in Portuguese | LILACS | ID: lil-436506

ABSTRACT

Avaliou-se o efeito da temperatura e do tempo de armazenamento sobre a contagem de células somáticas(CCS). Amostras de leite de 21 vacas, coletadas e armazenadas às temperaturas de 5, 27, 32 e 36°e e analisadas no 1°, 3°, 5°e 7° dia após coleta, foram classificadas em três grupos (células/ml): baixa (236.000 + ou - H64.000), média (624.000+ ou - 356.000) e alta CCS (1.320.000+ ou - 945.000). O delineamento experimental foi o de parcelas subdivididas. As médias da CCS foram iguais (P>0,05) para amostras mantidas à temperatura de 5°C e analisadas entre o primeiro e o sétimo dias após a coleta e para aquelas mantidas às temperaturas 5, 27, 32 e 36°e e analisadas no primeiro dia. Médias diferentes (P<0,05) da CCS foram observadas para amostras mantidas a 27, 32 e 36°e a partir do 5°, 5° e 3° dias, respectivamente. Foram observados decréscimos de 57,6%, 58,5% e 27,9% nos valores iniciais da CCS nas amostras classificadas como baixa, média e alta, respectivamente. Os resultados mostram a necessidade de se encaminhar as amostras de leite para CCS sob refrigeração e de ser feita análise até sete dias após a coleta.


Subject(s)
Cattle , Cell Count/methods , Milk/microbiology , Mastitis, Bovine/diagnosis
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