ABSTRACT
A fluorescence stereomicroscope system was developed in order to observe in situ the distribution of nuclei in intravascular Bong-Han ducts and corpuscle tissues by injecting acridine orange, which stained specifically nuclei. Intravascular Bong-Han corpuscles, connected with Bong-Han ducts could be detected in the aortas of rats, mice, and rabbits.
Subject(s)
Animals , Aorta/metabolism , Blood Vessels/pathology , Equipment Design , Mice , Mice, Inbred ICR , Microscopy/methods , Microscopy, Fluorescence/methods , Photons , Rabbits , Rats , Rats, Sprague-Dawley , Skin/metabolismABSTRACT
An in vivo method using Alcian blue (AB) was developed for visualizing floating threadlike tissues inside blood vessels of mice. These novel structures called intravascular Bonghan ducts (IBHDs) are considered as extension of acupuncture meridians. For in vivo imaging of IBHDs, AB solution (pH 7.4) that stains mucopolysaccharides like hyaluronic acid was used. After injecting AB solution into the femoral vein of a mouse, the threadlike structures, stained deep blue, inside the inferior vena cava. The histological results, such as hematoxylin, eosin, and AB staining, show the compositions of the cells and the extracellular matrix in the IBHD. Further studies are needed to investigate their physiological functions, especially in relation with those of other circulatory systems.
Subject(s)
Alcian Blue/pharmacology , Animals , Biophysics/methods , Blood Vessels/metabolism , Eosine Yellowish-(YS)/pharmacology , Femoral Vein/pathology , Hematoxylin/pharmacology , Hyaluronic Acid/chemistry , Hydrogen-Ion Concentration , Male , Mice , Mice, Inbred BALB C , Microscopy, Confocal , Microscopy, Phase-Contrast , Propidium/pharmacologyABSTRACT
The photon counting statistics of biophotons emitted from hands is studied with a view to test its agreement with the Poisson distribution. The moments of observed probability up to seventh order have been evaluated. The moments of biophoton emission from hands are in good agreement while those of dark counts of photomultiplier tube show large deviations from the theoretical values of Poisson distribution. The present results are consistent with the conventional delta-value analysis of the second moment of probability.
Subject(s)
Hand , Humans , Photons , Poisson Distribution , ProbabilityABSTRACT
Left-right biophoton asymmetry from the palm and the dorsum of hands from 7 Korean hemiparesis patients were studied. There is a strong tendency that the left-hemiparesis patients emit more biophotons from the right than the left hands, while the right-hemiparesis patient emits more from the left hand. Acupuncture treatment reduces dramatically the left-right asymmetry of biophoton emission rates. However there is no systematic difference for the patients in the emission rates from the palm and the dorsum of hands.