Simultaneous spectrophotometric determination of tramadol and acetaminophen in pharmaceutical formulationsusing H-point standard addition method

H-point standard addition method [HPSAM] was applied to the simultaneous spectrophotometric determination of tramadol and acetaminophen in pharmaceutical formulations. The results showed that tramadol and acetaminophen can be determined simultaneously in the range 5.0-100 and 1.0-10 microg/ml, respectively. The limits of detection [LOD] for tramadol and acetaminophen were obtained 2.0 and 0.50 microg/ml, respectively. Relative standard deviation for five replicate simultaneous determinations of tramadol and acetaminophen were calculated to be 3.2 and 3.3%, respectively. The proposed method has been successfully applied to the simultaneous determination of tramadol and acetaminophen in some synthetic, pharmaceutical formulation