Effect of ultrasound on the ultrastructure of rat skin and on the concentration of zinc and iron

This work deals with the use of ultrasound waves of frequency 20 KHz and average intensity 1.5 W/Cm[2], to study its effect on the cellular ultrastructure and the concentration of the zinc and iron on the rat skin. This study was conducted on 20 rats. They were divided into four groups: control, and three experimental groups exposed to ultrasound radiation for 15, 20 and 30 minutes respectively. Skin samples were taken to study thefr ultrastructure by a transmission electron microscope and their zinc and iron content by an atomic absorption spectrophotometry. There were evident on ultrastructural changes in rat skin, exposed to ultrasound at different intervals when compared with the normal tissue. As regard to the trace elements there was a significant decrease in concentration of Zn and increase in Fe concentration in comparison with the control group

Efficacy of praziquantel against Giardia lamblia in rats: parasitological, pathological and therapeutic study

Four-five weeks old rats were included in this study and divided into two experimental groups received single or split doses of praziquantel [PZQ] as well as two control groups, one infected untreated and the other normal healthy rats. The effect of infection on rat growth as well as the jejunal and duodenal architecture was histopathologically studied after H and E staining. The jejunal ultrastructure was examined by SEM and TEM. The effect of PZQ was evaluated using the same techniques. It was observed that infected as well as infected treated animals had less weight than the healthy controls. The intensity of infection decreased gradually after treatment. The cure rate was 100% after split dose and 80% after a single dose. Altered villus height and cryptic depth were the characteristic changes in the architecture of the duodenum and jejunum, more pronounced in the latter. The split dose of PZQ revealed a more improvement of the histopathological findings than the single dose. By SEM, circular imprints representing defects in the villi were observed in the jejunum. By TEM deformation of microvillar architecture was observed together with organellar changes in the RER and the mitochondria after PZQ treatment