ABSTRACT
Background: Extra-pulmonary tuberculosis (EPTB) remains a major problem in developing countries, which acquire diagnostic and management challenges. The study was conducted to characterize the diagnosed EPTB cases, and to understand the perspectives of clinicians on diagnosis and challenges in its management. Methods: A total of 150 EPTB patients' medical records were reviewed at two tertiary hospitals in Bengaluru, Karnataka for the period January 2017 to December 2017. The questionnaire-based interview was conducted among clinicians (n=42) during the study period. Results: The mean age and weight of EPTB patients (females (77, 51%) and males (73, 49%) were 32.5 (±17.5) years and 44.5 (±16.3) kilograms, respectively. Pleural TB (44, 29%), cervical lymph node TB (LNTB) (32, 21%) and abdominal TB (17, 11%) were the most common sites of EPTB involvement, followed by bones and joints TB (11, 7%), spine TB (10, 7%), hilar/mediastinal TB (9, 6%), tuberculoma (8, 5%), TB meningitis (6, 4%), abdominal LNTB (6, 4%), urinary TB (4, 3%) and others (3, 2%). We found GeneXpert was the basis of EPTB diagnosis in 43.3% of cases followed by acid-fast bacilli (AFB) smear microscopy (20.7%) and culture (12.5%). Of 150 EPTB suspected cases, 73% (110) were started anti-TB treatment (ATT), including 8% (12) having received ATT previously. According to LIKERT scale options, 90% of clinicians agreed GeneXpert as a promising diagnostic tool for diagnosis as compared to other microbiological (79%) and histopathological tests (55%). Conclusions: The quality of EPTB diagnosis and management practices at these institutions is satisfactory according to the principles laid down in INDEX TB guidelines or the national guidelines.
ABSTRACT
Dehydration in non-diarrheal illnesses often goes unnoticed and there are no consensus treatment recommendations for management of dehydration in non-diarrheal illnesses. A multi-disciplinary committee of 10 experts from India gathered on virtual platform in September 2020 to develop consensus recommendation on current treatment strategies for managing oral fluid electrolyte and energy in pediatric patients during non-diarrheal illnesses and to identify unmet needs and gaps. A pre-meeting questionnaire-based voting system was adopted to reach consensus followed by a discussion between panel members. In absence of consensus, the topic was opened for debate to arrive at an aligned recommendation. Key clinical challenges include identifying dehydration in acute illnesses and recommending appropriate quantity and type of oral fluids based on symptom severity. This consensus statement provides guidance on management of dehydration in non-diarrheal illnesses including recommendation on oral fluid, electrolytes, and appropriate energy management in pediatric population. Oral fluid, electrolyte and energy supplementation were recommended based on symptoms in acute non-diarrheal illness increased insensible losses and/or decreased intake. Oral rehydration fluids should be prescribed accurately and at the right-time for countering dehydration, ideally early in the course of illness. Prescribing pattern should be precise and like intravenous fluids. Carbonated drinks and canned juices should not be recommended. Plain water may not be optimal in replacing electrolytes especially for anorexic patients who can only tolerate fluids. These clinical practice statements provide guidance for oral fluid, electrolytes, and energy recommendations for pediatric patients with various acute illnesses beyond diarrhea.
ABSTRACT
Background/Aims@#Endoscopic visualization of the microscopic anatomy can facilitate the real-time diagnosis of pancreatobiliary disorders and provide guidance for its treatment. This study aimed to review the technique, image classification, and diagnostic performance of confocal laser endomicroscopy (CLE). @*Methods@#We conducted a systematic review of CLE in pancreatic and biliary ducts of humans, and have provided a narrative of the technique, image classification, diagnostic performance, ongoing research, and limitations. @*Results@#Probe-based CLE differentiates malignant from benign biliary strictures (sensitivity, ≥89%; specificity, ≥61%). Needlebased CLE differentiates mucinous from non-mucinous pancreatic cysts (sensitivity, 59%; specificity, ≥94%) and identifies dysplasia. Pancreatitis may develop in 2-7% of pancreatic cyst cases. Needle-based CLE has potential applications in adenocarcinoma, neuroendocrine tumors, and pancreatitis (chronic or autoimmune). Costs, catheter lifespan, endoscopist training, and interobserver variability are challenges for routine utilization. @*Conclusions@#CLE reveals microscopic pancreatobiliary system anatomy with adequate specificity and sensitivity. Reducing costs and simplifying image interpretation will promote utilization by advanced endoscopists.
ABSTRACT
Background/Aims@#Gastroparesis is a chronic gastrointestinal disorder that frequently presents with symptoms that are difficult to manage, necessitating frequent hospitalizations. We sought to determine the predictors of early readmission due to gastroparesis based on etiology. @*Methods@#We identified all adults discharged with a principal diagnosis of gastroparesis after hospitalization from the 2014 Nationwide Readmission Database. We compared etiology wise (diabetes, post-surgical, and idiopathic) early readmission. Multivariate regression analyses were performed to identify significant predictors of 30-day readmission. @*Results@#A total of 12 689 patients were identified, 30.7% diabetic, 2.6% post-surgical, and 66.7% were idiopathic. Patients with diabetic gastroparesis were more likely to be readmitted within 30 days than idiopathic (adjusted odds ratio [aOR], 0.81; 95% confidence interval [CI], 0.69-0.94) and post-surgical gastroparesis (aOR, 0.58; 95% CI, 0.34-0.98). Pyloroplasty was associated with less likelihood of 30-day readmission (aOR, 0.45; 95% CI, 0.20-0.97). In addition, male gender (aOR, 1.18; 95% CI, 1.02-1.37), modifiedElixhauser comorbidity score ≥ 3 (aOR, 1.38; 95% CI, 1.18-1.61), chronic pain syndrome (aOR, 1.41; 95% CI, 1.11-1.78), younger(18-64 years) age (aOR, 1.64; 95% CI, 1.34-2.00), need for percutaneous endoscopic gastrostomy/jejunostomy tube (aOR, 2.06; 95% CI, 1.21-3.52), and need for total parenteral nutrition (aOR, 1.70; 95% CI, 1.24-2.35) were associated with increased risk of 30-day readmission. @*Conclusions@#One in 5 patients was readmitted with gastroparesis within 30 days. In the diabetic group, diabetes-related complications contributed to readmissions than gastroparesis. Pyloroplasty is associated with reduced early hospital readmission. Prospective studies are needed for validation of these results.
ABSTRACT
Background/Aims@#Gastroparesis is a chronic gastrointestinal disorder that frequently presents with symptoms that are difficult to manage, necessitating frequent hospitalizations. We sought to determine the predictors of early readmission due to gastroparesis based on etiology. @*Methods@#We identified all adults discharged with a principal diagnosis of gastroparesis after hospitalization from the 2014 Nationwide Readmission Database. We compared etiology wise (diabetes, post-surgical, and idiopathic) early readmission. Multivariate regression analyses were performed to identify significant predictors of 30-day readmission. @*Results@#A total of 12 689 patients were identified, 30.7% diabetic, 2.6% post-surgical, and 66.7% were idiopathic. Patients with diabetic gastroparesis were more likely to be readmitted within 30 days than idiopathic (adjusted odds ratio [aOR], 0.81; 95% confidence interval [CI], 0.69-0.94) and post-surgical gastroparesis (aOR, 0.58; 95% CI, 0.34-0.98). Pyloroplasty was associated with less likelihood of 30-day readmission (aOR, 0.45; 95% CI, 0.20-0.97). In addition, male gender (aOR, 1.18; 95% CI, 1.02-1.37), modifiedElixhauser comorbidity score ≥ 3 (aOR, 1.38; 95% CI, 1.18-1.61), chronic pain syndrome (aOR, 1.41; 95% CI, 1.11-1.78), younger(18-64 years) age (aOR, 1.64; 95% CI, 1.34-2.00), need for percutaneous endoscopic gastrostomy/jejunostomy tube (aOR, 2.06; 95% CI, 1.21-3.52), and need for total parenteral nutrition (aOR, 1.70; 95% CI, 1.24-2.35) were associated with increased risk of 30-day readmission. @*Conclusions@#One in 5 patients was readmitted with gastroparesis within 30 days. In the diabetic group, diabetes-related complications contributed to readmissions than gastroparesis. Pyloroplasty is associated with reduced early hospital readmission. Prospective studies are needed for validation of these results.
ABSTRACT
A 22 year old primigravida with full term pregnancy with morbid obesity and short stature presented to us with complaints of labour pains six hours prior to admission. Her dating, anomaly and term growth scan showed a single live intrauterine foetus with foetal heart rate, amniotic fluid and growth parameters corresponding to the gestational age with no significant anomalies and normal adnexa.Following a thorough examination and pelvic assessment, diagnosis of cephalopelvic disproportion was made and the patient was posted for emergency caesarean section. A healthy male baby of weight 3.5 Kg extracted and the placenta and membrane were expelled in toto. Uterus was closed in double layer. On examination of adnexal structures, a right sided ovarian mass of around 10 x 8 x 6 cms of size, solid in consistency and proliferative in appearance was found. Suspecting it to be a malignancy and due to the unavailability of frozen section facility, a right sided oophorectomy was performed, and the specimen was sent for histopathological reporting which revealed a diagnosis of pregnancy luteoma of ovary
ABSTRACT
Haemolysis (H), elevated liver enzymes (EL) and low platelet count (LP) i.e., HELLP syndrome is a vaguely understood condition of pregnancy which can present with rapid onset. It is commonly associated with pre - eclampsia. HELLP is also known to manifest itself without the clinical features of pre - eclampsia. The present study aims to assess the maternal and foetal complications associated with HELLP syndrome. METHODSThis retrospective study included all the pregnant women who developed HELLP / partial HELLP with gestational age of ≥ 28 weeks. The variables analyzed were obstetric history, menstrual history, antenatal complications, laboratory investigations (haemolysis, ALT / AST, LDH, CBC), mode of delivery, postnatal complications, maternal outcomes and perinatal outcomes. RESULTS72 patients were included in the present study. The mean age of pregnant women with HELLP syndrome was 23.6 ± 4.15 years. The average age of gestation was 33.17 ± 4.02 weeks. 58 % patients were primigravida. As per Mississippi triple-class system 82 % patients had partial HELLP, 18 % had complete HELLP. 4 %, 4 % & 10 % patients had HELLP class I, II & III respectively. Among the total cases, 74 % patients had antepartum onset, 10 % had intrapartum & 17 % postpartum onset of HELLP syndrome respectively. 65 % patients delivered vaginally & rest 35 % underwent caesarean section. High risk factors such as pre - eclampsia (65 %), eclampsia (3 %) & previous history of HELLP (8 %) were noted in study cases. Abruptio placentae (18 %), postpartum haemorrhage (17 %), pulmonary oedema (14 %), renal failure (14 %) & DIC (7 %) were the maternal complications noted. Maternal mortality was 7 %. The major perinatal morbidities noted were prematurity (67 %) & FGR (42 %). Intrauterine death was noted in 19 % babies. Neonatal intensive care (NICU) was required for 58 % babies, of which 42 % had respiratory distress. Neonatal death was noted in 17 %. CONCLUSIONSHELLP syndrome is a life threatening condition of pregnancy which has serious maternal and perinatal morbidities. Prompt referral, timely and appropriate interventions can save lives. Availability of Intensive Care Units (ICU) facilities, dialysis units and blood and its components along with Neonatal Intensive Care Unit (NICU) facilities can remarkably reduce the maternal and neonatal complications.
ABSTRACT
Background: The pattern of prescribing anti-diabetic drugs varies among different medical professionals due to the availability of numerous anti-diabetic drugs and demographic and have increasingly favoured for tighter control of glycemic index. Even with the available multiple therapies many patients are not in control. The reasons may be multi factorial and the one important factor is the drugs prescribed.Methods: This cross-sectional study involving 140 type 2 adult diabetes patients was done to assess the pattern of anti-diabetic drugs prescribed by the postgraduate students in a tertiary health care center using a specially designed questionnaire.Results: Oral medications were predominantly prescribed. Metformin and Glimepiride combination was prescribed in majority. Among insulin, combination of short and intermediate acting insulin was prescribed more and the number of insulin prescription increased with the duration of diabetes. Interestingly no newer classes of drugs are prescribed.Conclusions: There is a rational prescription pattern of antidiabetic drugs but newer antidiabetic drugs are not prescribed and life style changes are not discussed with the patients. There is a need to include life style changes as a part of prescription to all the patients.
ABSTRACT
Background: Parenting styles are the methods used by parents when dealing with their children. Some theorists in counseling and psychotherapy have shown the importance of parenting styles in forming children personalities. Baumrind recommended three prototypes of parenting styles: authoritative, authoritarian, and permissive. Parenting styles are associated with different levels of social and cognitive competence in children and adolescents. Hence with this background the following study was undertaken to understand the preferred parenting style practiced in the rural community of southern part of India. The objective of this study is to assess the parenting style practiced in the rural community and to assess the association of socio-demographic variables with the type of parenting styles practiced in rural community.Methods: It is a cross-sectional study, conducted in Basavanapura village of Hunasuru taluk of Mysuru district. 141 parents were selected by simple random sampling. Data was collected using parenting style questionnaire (PSQ). The questionnaire also included information on socio demographic variables. Data was analyzed using SPSS V.24. Analysis is done using descriptive statistics like percentage and inferential statistics like chi square analysis to find the association between socio demographic variables and preferred parenting styles.Results: The most preferred parenting style reported was authoritative (63.2%) followed by authoritarian (26.2%) and permissive (10.6%) types. Years of marriage was found to be significantly associated with preferred parenting styles.Conclusions: Nearly three fourth of the parents practiced authoritative parenting style with this type of parenting style adopted by parents having children less than twenty years.
ABSTRACT
Pancreatic malignancy is the third leading cause of cancer related death in the United States with limited viable screening options. By the end of this decade, cancers are poised to become the leading cause of death with pancreatic cancer projected to be the second leading cause of cancer related mortality. Pancreatic cystic lesions (PCLs) are found in approximately 5%–14% of patients due to the increased utilization of cross-sectional imaging, with approximately 8%–10% of pancreatic cancers originating as PCLs. Current screening guidelines have shown discrepancies between morphologic characteristics of PCLs and identifying advanced pancreatic disease. Molecular analysis has emerged as a novel technology to aid in adequate diagnosis and management decisions of PCLs. Mucinous cysts including intraductal papillary mucinous neoplasms (IPMNs) or mucinous cystic neoplasms have similar oncogenic mutations including KRAS, TP53, SMAD4, PIK3CA, PTEN, or CKDN2A, while GNAS and RNF43 mutations are specific only to IPMNs. Serous cystadenomas have been associated with a loss of tumor suppressor gene VHL, while solid-psuedopapillary neoplasms have an oncogenic mutation CTNNB1. A specific molecular marker to diagnose existing high-grade dysplasia or impending malignant transformation is yet to be identified. Moving forward it is important to advance technology in isolating and identifying high-risk molecular markers from cyst fluid while considering their increased utilization in the evaluation of PCLs.
Subject(s)
Humans , Biomarkers, Tumor , Cause of Death , Cyst Fluid , Cystadenoma, Serous , Diagnosis , Genes, Tumor Suppressor , Loss of Heterozygosity , Mass Screening , Mortality , Mucins , Neoplasms, Cystic, Mucinous, and Serous , Pancreatic Cyst , Pancreatic Diseases , Pancreatic Neoplasms , United StatesABSTRACT
Background: The objective was to evaluate and compare the effect of an extract of essential oil of clove with the standard sodium valproate on pentylenetetrazole (PTZ) induced seizures in animal models. Methods: A total of 30 mice were taken, they were given a chemo shock at the concentration of 60 mg/kg using PTZ. 30 mice were divided into 5 groups of 6 animals each the control group received distilled water 5 ml/kg i.p., standard received injection sodium valproate 200 mg/kg i.p. another group received sesame oil – 10 ml/kg i.p. (control), test groups received Clove oil - 0.075 ml/kg i.p., clove oil - 0.1 ml/kg i.p., respectively. All the injections were given 30 mins before the administration of PTZ. Results: Clove oil produced a significant antiepileptic effect at all the doses. Conclusion: Clove oil has shown significant antiepileptic activity in mice.
ABSTRACT
Background: Tinea corporis is a common superficial dermatophytosis seen in tropical countries. This study was done to compare the efficacy and safety of topical antifungal agents, terbinafine versus sertaconazole in the treatment of tinea corporis. Methods: This study was conducted in Babuji Hospital and Chigateri Government Hospital, Davangere. A total of 60 (n=60) patients were included and divided into two groups of 30 patients each in the study. 1st group - received topical 1% terbinafine hydrochloride and 2nd group - received 2% sertaconazole cream. Patients were advised to apply cream twice daily for 4 weeks. Improvement in clinical parameters like erythema, scaling, itching, and potassium hydroxide (KOH) mount were taken for assessing the efficacy of drugs. They were followed-up at the end of 2, 4, and 6 weeks to assess the improvement of the condition. Complete cure rate was defined as mycological cure with a complete absence of clinical signs and symptoms. For safety data of both drugs presence of any local side effects (like stinging sensation, swelling and increased erythema) were analyzed in both groups. Statistical analysis was done using students paired t-test and unpaired t-test. Results: When two groups were compared at the end of 2 weeks, complete cure rate for terbinafine was 80% as compared to 63.3% for sertaconazole (p<0.003). However, at the end of 4 weeks, sertaconazole was as effective as terbinafine and statistically non-significant (p>0.05) results were obtained. KOH mount was negative in both groups at the end of 2nd week. Local side effects like erythema, swelling, stinging sensation were not noticed during the study in both the groups. Conclusion: From this study, it was shown that 2% sertaconazole cream was as effective as 1% terbinafine cream, though 1% terbinafine had higher rates of complete cure at the end of 2 weeks as compared to sertaconazole.
ABSTRACT
Background: The objective of thisstudy was to study the prescribing pattern of antipsychotic medications in patients with schizophrenia in a tertiary care hospital. Methods: An observational study was conducted in psychiatry outpatient department (OPD) of Bapuji and Chigateri hospital attached to Jagadguru Jayadeva Murugarajendra medical college, Davangere. The data collected, had information about age, gender, education, occupation, marital status and drug prescription that included trade name, generic name, dosage and frequency of administration of 100 outpatients who attended the psychiatry OPD. Results: A total of 100 prescriptions were analyzed of which 6% was monotherapy, where Asenapine was the only drug used and rest 94% was combination therapy. About 94% of the combination therapy included the use of single antipsychotic with anticholinergic drug and multiple antipsychotics with anticholinergic drug. A combination of multiple antipsychotics with anticholinergic was most commonly used, that is trifluoperazine and chlorpromazine (36%), followed by trifluoperazine and risperidone (13%), trifluoperazine and quetiapine (8%), risperidone and quetiapine (7%), trifluoperazine, chlorpromazine along with quetiapine (3%). Among single antipsychotic with anticholinergic, risperidone (20%) was most commonly used, followed by Trifluoperazine (7%). Use of the anticholinergic drug, Trihexyphenidyl accounts to 94% of the total prescriptions. Conclusions: Schizophrenia is mostly seen in males, middle age group and unemployed people. The present study showed that combination therapy is preferred for the treatment of Schizophrenia. Despite several side-effects, typical antipsychotics, especially trifluoperazine was the most commonly used drug, followed by chlorpromazine either alone or in combination. Among atypical antipsychotics, risperidone was commonly used followed by quetiapine and asenapine. Most of the patients received trihexyphenidyl, an anticholinergic drug along with antipsychotics to reduce extra pyramidal side-effects.
ABSTRACT
Background: The objective was to study the prescribing pattern of antidepressants in psychiatric unit of a tertiary care hospital. Methods: An observational study was carried out at psychiatry out-patient department (OPD). The data which were collected included information about age, gender, education, occupation, marital status and drug prescription included trade name, generic name, dosage, and frequency of 100 outpatients who attended the psychiatry OPD. Results: Among 100 patients with major depression 66% were females and 34% were males. Depression was more commonly seen between patients with age group 21-40 years. Depression was more common among housewives (44%) and next was students (18%). Percentage of depression was more in educated people with (72%) than in uneducated with (28%). Depression was more commonly seen in married people with (77%) than in unmarried people (23%). Most commonly prescribed antidepressant as monotherapy was fluoxetine and as combination therapy was fluoxetine and escitalopram. Conclusions: Depression is more commonly seen in married people predominantly in females and housewives. Fluoxetine is more commonly used followed by escitalopram. Selective serotonin reuptake inhibitors are preferred over other antidepressant because of their relative lesser side effects.
ABSTRACT
Objectives: To evaluate possible ocular hypotensive effect of 0.5% diltiazem and 0.1% verapamil eye drops on intraocular pressure in steroid induced glaucoma model of rabbits. And compare with 0.5% timolol eye drops. Methodology: Glaucoma was induced in rabbits (N=18) by bilateral topical instillation of 1% prednisolone eye drop (10 μl) twice a day for a period of 40 days. Before the induction of glaucoma, baseline intraocular pressure (IOP) in both the eyes of all rabbits was measured under sedation (i.v midazolam) by Schiotz tonometer. At the end of 40 days induced IOP was measured for all rabbits and rabbits were divided into three groups of six rabbits in each. Right eyes of group A, B and C rabbits received 0.5% diltiazem, 0.1% verapamil, and 0.5% timolol eye drops twice daily for 12 days respectively. Whereas, left eyes of all rabbits received distilled water hence represented as control. IOP was measured in all rabbits on every 4th day till 12 days of treatment period. Results: Intra-group comparisons of IOP changes were made by paired‘t’ test. And unpaired‘t’ test for inter group comparisons. One way ANOVA was used for multiple group comparisons followed by post-hoc Tukey’s test for group wise comparisons. In 0.5% diltiazem treated eyes, the mean IOP significantly reduced from 22.9±1.9 mmHg (10%) on 4th day to 16.9±1.1 mmHg(S, P<.001) on 12th day (34%). Similarly, mean IOP in 0.1% verapamil treated eyes significantly reduced from 22.7±1.3 mmHg (7%) on 4th day to 15.5±1.4 mmHg(S, P<.001) on 12th day (37%). Whereas, mean IOP significantly reduced from 22.4±1.9 mmHg (14%) on 4th day to 16.4±1.4 mmHg (S, P=.001) on 12th day (36%) in 0.5% timolol treated eyes. Conclusion: Topical 0.5% diltiazem and 0.1% verapamil significantly reduced the IOP in steroid induced glaucoma model of rabbits. However, Further research has to be carried out both in experimental and clinical subjects to reveal its efficacy and safety profile.
ABSTRACT
Mucoadhesive buccal tablets containing ondansetron hydrochloride (ODH) were prepared using polymers like gelatin, chitosan, xanthan gum in varying concentration of 5, 10, 15% w/w and HPMC K4M 40% w/w by direct compression technique. Precompressional studies revealed good micromeritic properties of powder blend for compression and were found as per literature limits. The prepared tablets were evaluated for thickness, hardness, uniformity of weight, drug content, friability, swelling index, mucoadhesion strength, in vitro disintegration, dissolution time and permeation studies. The formulations containing xanthan gum gave better mucoadhesion, release characteristics compared to those containing gelatin and chitosan and the rank order of mucoadhesion and permeation across sheep buccal mucosa was xanthan gum > chitosan> gelatin. The tablets apart from fulfilling all the official specifications, exhibited higher rate of release, in vitro release from all ODH buccal tablets followed Super case II transport due to polymer chain disentanglement and relaxation. and found to be stable upon conducting stability studies as per ICH guidelines at 40ºC/75 % RH. The results revealed that mucoadhesive buccal tablets containing ODH were successfully formulated by direct compression technique as an alternative to conventional tablets for therapy of nausea condition in patients.
ABSTRACT
Static, short-term, acute toxicity tests were performed over a period of 96 hrs using different concentrations of influent and effluent of textile industry waste water with the objective of evaluating their acute toxicity on fresh water fish, Cyprinus carpio (common carp). The LC50 24, 48, 72 and 96 hr of influent and effluent were 25.9, 21.10, 15.66, 11.11% (v/v) and 63.18, 54.89, 48.62, 36.04% (v/v), respectively. The acute toxic unit TUa values for 24, 48, 72, 96 hr for influent and effluent are 3.85, 4.73, 6.38, 8.99 and 1.58, 1.82, 2.05, 2.77, respectively. Correspondingly, the TF was found to be 1, 1.22, 1.65 and 2.33 for influent, and for effluent 1, 1.15, 1.29 and 1.75. Total efficiency of the treatment was 69.16% and the safe concentration of effluent is set to be 3.60%. These data are highly useful in establishing limits of acceptability by the aquatic animals. The need to introduce toxicity evaluation assay for confirming the quality of effluent from the point view of effective environmental safe limits and to ensure integrity of aquatic environment, is stressed.
ABSTRACT
Budesonide is a very potent corticosteroid, used for bronchial asthma and inflammatory bowel disease. Objective of the present investigation is to develop the simple and selective UV spectrophotometric method for quantification of budesonide in bulk sample. Absorption maximum of budesonide was found to be 246.0 nm and obeyed the beers law in the concentration range of 1.4 to 25 μ g/ml. Calibration curve shows a linear relationship between the absorbance and concentration in the range of 2 to 10 μ g/ml and the limit of detection is 0.01 μ g/ml. The limit of quantification was found to be 1.4 μg/ml. The method was validated for repeatability, accuracy and precision. The percent amount of recovery was 99 - 100% with minimum standard deviation less than 1%. Obtained results showed there is minimum intra day and inter day variation. The excipients present in the preparation did not interfered during the analysis. Developed analytical UV spectrophotometric method is simple, rapid and reproducible and further it can be used for estimation of drug in bulk and colon matrix tablet dosage form.
ABSTRACT
Background: The glutamate system has been implicated in depression recently. This is a departure from previous thinking, which had focused on serotonin and norepinephrine. The glutamate system may represent a new avenue for treatment and research. NMDA and AMPA are receptors for the neurotransmitter glutamate. Blocking NMDA increases the activity of another receptor, AMPA, and this boost in AMPA activity is crucial for rapid antidepressant actions. Amantidine being a noncompetitive antagonist at NMDA receptor is evaluated for its antidepressant activity in this study. Objectives: To evaluate the antidepressant activity of amantidine and compare it with Imipramine in albino mice. Methodology: Total of 18 swiss albino male mice were used. They were divided into three treatment groups and with normal saline (control) 10mg/kg, Imipramine (standard) 10mg/kg and amantidine 26 mg/kg (test drug) given orally. Each group contained 6 animals. Duration of immobility was observed for 6 minutes in tail suspension test and for 4 minutes in forced swimming test on separate set of animals. Results: Results were analyzed by ANOVA followed by Post hoc Tukey’s test. Amantidine at the dose of 26 mg/kg significantly reduced the immobility time in both the tests compared to control (p < 0.05). Conclusion: Non-competative antagonist, amantidine has significant antidepressant activity in acute models of depression.
ABSTRACT
Fire is the most spectacular natural disturbance that affects the forest ecosystem composition and diversity. Fire has a devastating effect on the landscape and its impact is felt at every level of the ecosystem and it is possible to map forest fire risk zone and thereby minimize the frequency of fire. There is a need for supranational approaches that analyze wide scenarios of factors involved and global fire effects. Fires can be monitored and analyzed over large areas in a timely and cost effective manner by using satellite imagery. Also Geographical Information System (GIS) can be used effectively to demarcate the fire risk zone map. Bhadra wildlife Sanctuary located in Karnataka, India was selected for this study. Vegetation, slope, distance from roads, settlements parameters were derived for a study area using topographic maps and field information. The Remote Sensing (RS) and Geographical Information System (GIS)-based forest fire risk model of the study area appeared to be highly compatible with the actual fire-affected sites. The temporal satellite data from 1989 to 2006 have been analyzed to map the burnt areas. These classes were weighted according to their influence on forest fire. Four categories of fire risk regions such as Low, Moderate, High and Very high fire intensity zones were identified. It is predicted that around 10.31% of the area falls under moderate risk zone.