[GSK3beta phosphorylation with DHEA in neural progenitor cells derived from Balb/c mouse embryos brain]

Studies have shown that any disruption in wnt signaling pathway is associated with Alzheimer disease [AD]. One of the important molecules involved in activation or inactivation of this pathway is GSK3beta [glycogen syntase kinase3beta]. The main goal of this study was to evaluate GSK3beta phosphorylation by treatment of cells with dihydroepiandrosterone [DHEA], a kind of neurosteroid that decreases in the brain with aging. In this experimental study, neural progenitor cells were obtained from mouse embryos brain. Then, these cells were treated with 1microm concentration of DHEA for 48h. After 48h, the phosphorylation of GSK3beta was analyzed by immunocytochemistry. DHEA increased phosphorylation of GSK3beta in neural cells treated by DHEA, whole in control group, we could not detect the expression of GSK3beta. DHEA can increase phosphorylation of GSK3beta in neural cells and inactivation of GSK3beta can help to cure AD

Derivation of adipocytes from human endometrial stem cells [EnSCs]

Due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. In this study, we investigated the ability of Human Endometrial-derived stem cells [EnSCs] as a new source of mesenchymal stem cells to differentiate into adipocytes. EnSCs are the abundant and easy available source with no immunological response, for cell replacement therapy. Single-cell suspensions of EnSCs were obtained from endometrial tissues from 10 women experiencing normal menstrual cycles, and were cultured at clonal density [10 cells/cm[2]] or limiting dilution. Endometrial mesenchymal stem cell markers were examined flow cytometry. These cells were treated with adipogenicinducing medium for 28 days. The adipogenic differentiation of the EnSC was assessed by cellular morphology and further confirmed by Oil Red O staining and RTPCR. The BM-MSC differentiated into adipocytes in the presence of adipogenic stimuli for 3 weeks. The flow cytometric analysis showed that the cells were positive for CD90, CD105, CD146 and were negative for CD31, CD34.We showed that the key adipocytes marker PPARa was expressed in mRNA level after 28 days post treatment [PT]. According to our finding, it can be concluded that EnSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage